Kusanthula kwa njira zopangira ma peptide

Tekinoloje ya synthesis ya peptide

Kafukufuku ndi chitukuko cha mankhwala a peptide akukula mofulumira mu mankhwala.Komabe, kukula kwa mankhwala a peptide kumachepa ndi mawonekedwe awo.Mwachitsanzo, chifukwa cha chidwi chapadera cha enzymatic hydrolysis, kukhazikika kumachepetsedwa, ndipo kusinthasintha kwa steric conformation kumabweretsa kutsika kwachindunji, kutsika kwa hydrophobicity, komanso kusowa kwa kayendedwe kake.Kuti mugonjetse ma peptide awa, mayankho ambiri omwe adaperekedwa komanso kugwiritsa ntchito bwino mtundu umodzi wa peptide ndi amodzi mwa iwo.

Mtundu wa peptide (dzina lachingerezi: Peptoid) kapena Poly - N - m'malo mwa glycine (dzina la Chingerezi: Poly real - N - substitutedglycine), ndi quasi peptide compounds of peptide in main chain.Unyolo wa mbali ya alpha carbon umasamutsa nayitrogeni waukulu m'malo mwa unyolo wam'mbali.Mu polypeptide yoyambirira, gulu la R la mbali ya amino acid limayimira ma amino acid 20, koma gulu la R lili ndi zosankha zambiri mu peptoid.Mu peptide, peptide pa unyolo waukulu wa amino zidulo mu alpha carbon nayitrogeni m'malo mwa mbali unyolo kusamutsira ku unyolo waukulu.Ndikoyenera kutchula kuti ma peptides ambiri samapanga mapangidwe apamwamba omwe amalamulidwa monga zigawo zachiwiri mu peptides ndi mapuloteni chifukwa cha kusowa kwa haidrojeni pamsana wa nayitrogeni.Cholinga choyambirira cha peptide ndikupanga peptide yokhazikika komanso ya proteinase yamankhwala ang'onoang'ono a molekyulu.

Kusanthula kwa njira zopangira ma peptide

Njira yopangira peptide synthesis idayambitsidwa

Njira yodziwika bwino ya peptide ngati kaphatikizidwe kake ndi njira yocheperako yopangidwa ndi RonZuckermann, yomwe ili ndi magawo awiri: acylation ndi kusamuka.Mu acylation, sitepe yoyamba ndikuyambitsa haloacetic acid kuti igwirizane ndi ma amine omwe atsala kumapeto kwa sitepe yapitayi, nthawi zambiri diisopropyl carbonized diimine.Bromoacetic acid idayambitsidwa ndi diisopropylcarbodiimide."Mu substitution reactions (bimolecular nucleophilic substitution reactions), amine, makamaka choyambirira, amaukira m'malo mwa halogen kupanga N-substituted glycine."Njira ya subunitary synthetic imagwiritsa ntchito ma amine oyambira omwe amapezeka mosavuta kuti apange ma peptides, potero amathandizira kupanga mankhwala a peptides.

Kukula kolimba mu kaphatikizidwe ka ma peptide amkalasi kumakhala ndi zambiri, kumatha kukupatsirani mitundu yosiyanasiyana ya ntchito za peptide synthesis.

Kusanthula kwa njira zopangira ma peptide

Ubwino wa peptide yotere

Okhazikika: ma peptoids amakhala okhazikika mu vivo kuposa ma peptides.

Kusankha kowonjezereka: Peptoids ndi yoyenera pamaphunziro ophatikizana opeza mankhwala chifukwa mitundu ingapo yamitundu yosiyanasiyana yomangira ya polypeptide imatha kupezeka posintha gulu la amino la msana.

Kuchita bwino kwambiri: Kuchuluka kwa mapangidwe a peptoid kungapangitse peptoid kukhala chisankho chabwino pakusanthula njira kuti ipeze mwachangu zida zomwe zimalumikizana ndi mapuloteni.

Kuthekera kochulukirapo kwa msika: mawonekedwe amtundu wa peptide amalola kuti akhale mtundu wa chitukuko cha mankhwala ali ndi kuthekera kwakukulu.