Chiwembu chopanga ndi yankho la unyolo wa polypeptide peptide

I. Chidule
Ma peptides ndi ma macromolecule apadera kotero kuti kutsatizana kwawo kumakhala kosazolowereka pamakina awo amthupi komanso mawonekedwe awo.Ma peptides ena ndi ovuta kupanga, pamene ena ndi osavuta kupanga koma ovuta kuwayeretsa.Vuto lalikulu ndilakuti ma peptides ambiri amasungunuka pang'ono munjira zamadzimadzi, kotero pakuyeretsedwa kwathu, gawo lofananira la hydrophobic peptide liyenera kusungunuka muzosungunulira zopanda madzi, Chifukwa chake, zosungunulira izi kapena ma buffers zitha kukhala zosagwirizana kwambiri ndikugwiritsa ntchito. za njira zoyesera zachilengedwe, kotero kuti akatswiri amaletsedwa kugwiritsa ntchito peptide pazolinga zawo, kotero kuti zotsatirazi ndizinthu zingapo zamapangidwe a peptides kwa ofufuza.

Chiwembu chopanga ndi yankho la unyolo wa polypeptide peptide
Chachiwiri, kusankha olondola a kupanga zovuta peptides
1. Kutalika konse kwa njira zotsatiridwa pansi
Ma peptides osakwana 15 zotsalira ndizosavuta kupeza chifukwa kukula kwa peptide kumawonjezeka ndipo kuyera kwa zinthu zopanda pake kumachepa.Pamene kutalika kwa unyolo wa peptide ukuchulukirachulukira kupitilira zotsalira 20, kuchuluka kwazinthu zenizeni ndizofunikira kwambiri.Muzoyesera zambiri, ndizosavuta kupeza zotsatira zosayembekezereka potsitsa nambala yotsalira pansi pa 20.
2. Chepetsani kuchuluka kwa zotsalira za hydrophobic
Ma peptides okhala ndi zotsalira zambiri za hydrophobic, makamaka m'chigawo cha 7-12 zotsalira kuchokera ku C-terminus, nthawi zambiri zimayambitsa zovuta zopanga.Izi zimawoneka ngati kuphatikiza kosakwanira ndendende chifukwa pepala la B-fold limapezeka pakuphatikiza."Zikatero, zitha kukhala zothandiza kutembenuza zotsalira ziwiri zabwino kapena zoyipa, kapena kuyika Gly kapena Pro mu peptide kuti mutsegule ma peptide."
3. Kuchepetsa kwa zotsalira "zovuta".
"Pali zotsalira zingapo za Cys, Met, Arg, ndi Try zomwe sizimapangidwa mosavuta."Ser idzagwiritsidwa ntchito ngati njira ina yopanda oxidative ku Cys.
Chiwembu chopanga ndi yankho la unyolo wa polypeptide peptide

Chachitatu, sinthani kusankha koyenera kwa sungunuka m'madzi
1. Sinthani poimilira N kapena C
Zokhudzana ndi ma peptides acidic (ndiko kuti, oyimbidwa molakwika pa pH 7), acetylation (N-terminus acetylation, C terminus nthawi zonse amakhala ndi gulu laulere la carboxyl) akulimbikitsidwa makamaka kuti awonjezere ndalama zoyipa.Komabe, pama peptides oyambira (ndiko kuti, oyimbidwa bwino pa pH 7), amination (gulu la amino laulere pa N-terminus ndi amination pa C-terminus) akulimbikitsidwa makamaka kuti awonjezere mtengo wabwino.

2. Kufupikitsa kapena kutalikitsa ndondomekoyi

Zina mwazotsatira zili ndi chiwerengero chachikulu cha hydrophobic amino acid, monga Trp, Phe, Val, Ile, Leu, Met, Tyr ndi Ala, etc. Pamene zotsalira za hydrophobic zimaposa 50%, nthawi zambiri zimakhala zosavuta kusungunuka.Zingakhale zothandiza kutalikitsa kutsatana kuti muwonjezere mizati yabwino komanso yoyipa ya peptide.Njira yachiwiri ndikuchepetsa kukula kwa tcheni cha peptide kuti muwonjezere mitengo yabwino ndi yoyipa pochepetsa zotsalira za hydrophobic.Kulimba kwa mbali zabwino ndi zoyipa za tcheni cha peptide, m'pamenenso zimatheka kuchitapo kanthu ndi madzi.
3. Ikani zotsalira zosungunuka m'madzi
Kwa maunyolo ena a peptide, kuphatikiza kwa ma amino acid abwino komanso oyipa kumatha kusintha kusungunuka kwamadzi.Kampani yathu imalimbikitsa N-terminus kapena C-terminus ya ma peptides acidic kuti aphatikizidwe ndi Glu-Glu.N kapena C terminus ya peptide yoyambira idaperekedwa kenako Lys-Ls.Ngati gulu loyimbidwa silingayikidwe, Ser-Gly-Ser imathanso kuyikidwa mu N kapena C terminus.Komabe, njirayi siigwira ntchito pamene mbali za peptide chain sizingasinthidwe.