Kapangidwe ka chiwembu ndi yankho la polypeptide peptide unyolo

I. Chidule
Peptides are special macromolecules such that their sequences are unusual in their chemical and physical features. Some peptides are difficult to synthesize, while others are relatively easy to synthesize but difficult to purify. Vuto lothandiza ndikuti ma peptive ambiri amasungunuka pang'ono m'matumba, kotero mu kuyeretsa kwathu, chifukwa chake, izi zimaletsedwa mosagwirizana ndikugwiritsa ntchito zilonda zake, kuti izi ndi zina mwazinthu zingapo of the design of peptides for researchers.

Kapangidwe ka chiwembu ndi yankho la polypeptide peptide unyolo
Chachiwiri, chisankho cholondola cha zopangidwa
1. Kutalika kwathunthu kwa magawo otsika
Peptinthus zosakwana 15 ndizosavuta kupeza chifukwa kukula kwa peputiyo kumawonjezeka ndipo kuyera kwa zinthu za kununkhira kumachepa. Pamene kutalika kwathunthu kwa peptide kumapitilira kupitirira zaka 20, zochulukitsa zochulukitsa ndizofunika kwambiri. Mu zoyeserera zambiri, ndizosavuta kupeza zovuta zosayembekezereka potsitsa nambala yotsalira pansipa 20.
2. Chepetsa kuchuluka kwa ma hydrophobic otsalira
Peptides yokhala ndi nthawi yayitali ya hydrophobic zotsalira, makamaka m'chigawo cha 7-12 zotsalira kuchokera ku C-tenemile, nthawi zambiri zimayambitsa kusangalatsa kochepa. Izi zimawoneka ngati zosakira zosakwanira chifukwa pepala la B lizipezeka mu synthesis. "Zikakhala zotere, zingakhale zothandiza kusintha zoposa ziwiri zabwino komanso zoyipa kapena kuyika papepala kuti itsegule mawonekedwe a peptide."
3. Kutsika kwa zotsalira "zovuta"
"Pali zigwa zingapo, zomwe zidakumana, arg, ndikuyesa zotsalira zomwe sizipangidwa mosavuta." Tizigwiritsa ntchito ngati njira yopanda ma cys.
Kapangidwe ka chiwembu ndi yankho la polypeptide peptide unyolo

Chachitatu, sinthani zosankha zolondola m'madzi
1. Sinthani n kapena c termisus
Wachibale ma peptives (ndiye kuti, akutsutsana ndi ma pH 7), acetylation (n-denguvu (N Komabe, pazithunzi zoyambira (ndiye kuti, zolipiridwa bwino pa PH 7), matanidwe (a Amino Gulu la Nino ku N-Cormisus ndi Kumizidwa ku C-Serminus) kumalimbikitsidwa kuti muchepetse mtengo wabwino.

2. Kufupikitsa kwakukulu kapena kukulitsa tsambali

Zina mwazotsatira zimakhala ndi kuchuluka kwa Hydrophobic Aacic Acids, monga Trp, phe, a Leu, etc. Nthawi zambiri zimakhala zosavuta kusungunuka. Zitha kukhala zothandiza kukulitsa gawo lotsatirali kuti muwonjezere mitengo yabwino komanso yolakwika ya peputiyi. Njira yachiwiri ndiyosaletsa kukula kwa utoto wa peptide kuti muwonjezere mitengo yotsimikizika komanso yoyipa pochepetsa zotsalira za hydrophobic. Cholinga champhamvu ndi chosalimbikitsa cha peptide utoto, nthawi zambiri zimagwirizana ndi madzi.
3. Ikani zotsalira zam'madzi zosungunuka
Kwa maunyolo ena pa Peptide, kuphatikiza kwa ma amino ma amino ma amino ma acid amatha kusintha madzi. Kampani yathu imalimbikitsa n-termisus kapena c-malemu a acidic toptic kuti aphatikizidwe ndi Glu-Glu. The N kapena C Trumkos ya peputiyi idaperekedwa kenako ma LYS. Ngati gulu lokhomera silingayikidwe, ser-gly-ser ikhoza kuyikidwanso mu n kapena c termisus. Komabe, njira imeneyi siyigwira ntchito pamene mbali za pepala la peptide sizingasinthidwe.