Kutumiza kwa Blurescence Kutumiza (Fret)
Fluorescence Resonance Exenes (Fret) ndi njira yosasinthika yopanda mphamvu yomwe Woperekayo adasamutsidwa ku boma lovomerezeka kudzera mu kulumikizana kwa mabanja apakatikati. Njirayi siyitenga Photons ndipo motero sikapanda phindu. Ukadaulo uwu uli ndi zabwino zokhala mwachangu, zowoneka bwino komanso zosavuta.
Utoto womwe umagwiritsidwa ntchito mu Fret Stuyey ikhoza kukhala yofanana. Koma mu ntchito zambiri, utoto wosiyanasiyana umagwiritsidwa ntchito kwenikweni. Mwachidule, kusamutsa kwamphamvu kwa kuwunika ndikosamutsa ma dipotsi kuchokera ku dongo kuchokera ku dongo (utoto 1) kupita ku Wovomereza (utoto 2) pomwe gulu la woperekayo ndi losangalala. Mwambiri, mawonekedwe a omwe adatulutsa Fluor fluoropep amapaka ndi mayamwidwe omwe amalandila gulu lovomerezeka. "Patali pakati pa ma fluoropengores awiri ndi oyenera (10 - 100 a), kusamutsa kwamphamvu kwa woperekayo kwa wovomereza kumatha kuonedwa." Njira yosinthira mphamvu zimatengera kapangidwe ka mankhwala olandilira:
1. Imasinthidwa kukhala kugwedezeka kwa molecular, ndiye kuti, kuunika koopsa kwa kusamutsa mphamvu kumatha. (Receptor ndi ndulu yowala)
2. Kutulutsa kumakhala kokulirapo kuposa retphory, zomwe zimapangitsa kukonzanso kwachiwiri kwa seluorescence. " (Receptors ndi okwera okwera).
Gulu la opereka (Edan) ndi Gener Gener (Dabcyl) ndi yolumikizidwa ndi protefam ya chilengedwe, ndipo gawo lapansi silikusakanizidwa, DABCYL itha kukhala yosawoneka bwino. Pakucheza kwa HIV-1, Edans sazimitsidwanso ndi DABCYL, ndipo advans a Luciferase amatha kupezeka pambuyo pake. Kupezeka kwa purtease zopinga kumatha kuyang'aniridwa ndi kusintha kwamphamvu kwa edent.
Fret Peptives ndi zida zosavuta kuphunzira za Peptidase. Popeza njira yake itha kuyang'aniridwa mosalekeza, imapereka njira yabwino yodziwitsira ntchito ya enzyme. Sheen yopangidwa pambuyo pa hydrolysis a peptide ndi wopereka / wolandila amapereka gawo la enzyme ntchito ku Nanomor yozungulira. Pamene Fret Peptide ndi yolimba, imawonetsa kuchepa kwadzidzidzi kwa chiwongola dzanja chamkati, koma pomwe peptide iliyonse yolumikizira dongo / wolandila, imatulutsanso phokoso mosalekeza ndipo imatha kupezeka mosalekeza.
Post Nthawi: 2025-07-02