Malinga ndi njira yolumikizira amino acid ndi shuga, peptide ya shuga imatha kugawidwa m'magulu anayi: O glycosylation, C a N glycosylation, mame saccharification ndi GPI (glycophosphatidlyinositol) kulumikizana.
1. N-glycosylation glycopeptides amapangidwa ndi N-acetamide glucose kumapeto kwa unyolo wa glycan (Glc-Nac) wolumikizidwa ndi atomu ya N pa gulu la amide la unyolo wam'mbali wa Asn ena mu unyolo wa peptide, ndi Asn. wokhoza kulumikiza unyolo wa glycan uyenera kukhala mu AsN-X-Ser / Thr (X! = P) mu motif yopangidwa ndi zotsalira.Shuga ndi N-acetylglucosamine.
N-glycosylation kusinthidwa structural glycopeptide
2. Kapangidwe ka O-glycosylation ndi kosavuta kuposa N-glycosylation.Glycopeptide iyi nthawi zambiri imakhala yayifupi kuposa glycan, koma ili ndi mitundu yambiri kuposa N-glycosylation.Ser ndi Thr amatha kukhala glycosylated mu unyolo wa peptide.Kuphatikiza apo, pali ma glycopeptides okongoletsedwa ndi tyrosine, hydroxyl, ndi hydroxyproline glycosylation.Malo olumikizirana ndi atomu ya okosijeni wa hydroxyl pa unyolo wam'mbali wa zotsalira.Shuga wolumikizidwa ndi galactose kapena N-acetylgalactosamine (Gal&GalNAc) kapena glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), etc.
O-glycosylation imasintha kapangidwe kake
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
N-acetylglucosamine (GlcNAc) glycosylation imodzi imagwirizanitsa mapuloteni O-GlcNAc ku atomu ya hydroxyl oxygen ya serine kapena threonine yotsalira ya mapuloteni.O-GlcNA glycosylation ndi O-GlcNAc monosaccharide chokongoletsera popanda glycan kutambasuka;Monga peptide phosphorylation, O-GlcNAc glycosylation ya glycopeptides imakhalanso njira yokongoletsera mapuloteni.Kukongoletsa kwachilendo kwa O-GlcNAc kungayambitse matenda osiyanasiyana monga shuga, matenda amtima, zotupa, matenda a Alzheimer ndi zina zotero.
Glycosylation mfundo za glycopeptides
Zomangamanga za polypeptide ndi unyolo wa shuga zimalumikizidwa ndi unyolo wa mapuloteni ndi ma covalent bond, ndipo masamba omwe amalumikiza maunyolo a shuga amatchedwa malo a glycosylation.Popeza palibe Chinsinsi kutsatira biosynthesis wa glycopeptide unyolo shuga, unyolo zosiyanasiyana shuga adzakhala Ufumuyo glycosylation malo, zikubweretsa otchedwa tosaoneka inhomogeneity.
Glycosylation ya glycopeptides
1. Zotsatira za glycopeptide glycosylation pa chithandizo chamankhwala chothandizira mapuloteni
Pankhani ya mapuloteni ochiritsira, glycosylation imakhudzanso theka la moyo komanso kulunjika kwa mankhwala a protein mu vivo.
2. Kusungunuka kwa glycopeptide glycosylation ndi mapuloteni
Kafukufuku wasonyeza kuti maunyolo a shuga omwe ali pamwamba pa mapuloteni amatha kusintha kusungunuka kwa mapuloteni
3. Glycopeptide glycosylation ndi mapuloteni immunogenicity
Kumbali imodzi, maunyolo a shuga omwe ali pamwamba pa mapuloteni angapangitse kuti chitetezo cha mthupi chitetezeke.Kumbali ina, unyolo wa shuga ukhoza kuphimba malo ena pamwamba pa mapuloteni ndikuchepetsa chitetezo chake
4. Glycopeptide glycosylation yomwe imawonjezera kukhazikika kwa mapuloteni
Glycosylation imatha kukulitsa kukhazikika kwa mapuloteni kumayendedwe osiyanasiyana (monga ma denaturants, kutentha, ndi zina) ndikupewa kuphatikizika kwa mapuloteni.Nthawi yomweyo, maunyolo a shuga omwe ali pamwamba pa mapuloteni amathanso kuphimba malo ena owonongeka a proteinolytic a mamolekyu a protein, potero kumawonjezera kukana kwa mapuloteni ku proteinases.
5. Glycopeptide glycosylation yomwe imakhudza ntchito yachilengedwe ya mamolekyu a mapuloteni
Kusintha mapuloteni a glycosylation kungathandizenso kuti mamolekyu a mapuloteni apange zochitika zatsopano zamoyo
Nthawi yotumiza: Aug-03-2023