Momwe mungapangire RGD cyclopeptide

Integrin, kapena integrin, ndi heterodimer transmembrane glycoprotein receptor yomwe imayimira kumamatira kwa maselo a nyama ndi chizindikiro.Amapangidwa ndiα ndiβ magulu.Zimakhudzidwa ndi kukhathamiritsa kwa machitidwe osiyanasiyana a ma cell kuphatikiza kusamuka kwa ma cell, kulowa mkati mwa cell, ma cell ndi ma intercellular signing, cell adhesion, ndi angiogenesis.Integrinαvβ3 tsopano ikufufuzidwa kwambiri.Mawonekedwe a integrinαvβ3 imagwirizana kwambiri ndi kusamuka kwa chotupa, angiogenesis, kutupa ndi kufooka kwa mafupa.Integrin imawonetsedwa kwambiri muzotupa zonse za chotupa ndi endothelial cell neovascularization.Maonekedwe a integrin amagwirizana kwambiri ndi kusamuka kwa chotupa ndi angiogenesis.M'zaka zaposachedwa, kafukufuku wasayansi awonetsa kuti pali ma integrins 11 omwe amatha kumangirira makamaka ku RGD peptide, omwe ndi ma peptides otsutsa a integrin receptors.

RGD peptide imayikidwa mu mzere wa RGD peptide ndi RGD cyclic peptide.Poyerekeza ndi mzere wa RGD peptide, RGD cyclic peptide ili ndi kuyanjana kwamphamvu kwa ma receptor komanso kutsimikizika kwa ma receptor.Zotsatirazi ndi mitundu wamba ndi kaphatikizidwe njira RGD cyclic peptide.

Mitundu yodziwika bwino ya RGD cyclic peptides:

1. Ma cyclic peptides okhala ndi ma RGD amatsatizana opangidwa ndi ma disulfide bond

2. Ma cyclic peptides okhala ndi ma RGD amatsatizana opangidwa ndi ma amide bond

Kaphatikizidwe ka RGD cyclic peptide:

Mtundu wogwiritsidwa ntchito umakhudzana ndi njira ya RGD cyclic peptide synthesis m'munda waukadaulo wokhazikika wa polypeptide synthesis.Njira yatsopano ndikusankha utomoni wa 2-chloro-triphenylmethyl chloride monga chonyamulira chofunikira, choyamba kulumikiza gulu loyamba la mbali ya carboxyl ndi gulu lapadera loteteza la D aspartic acid amino acid, kenako ndikulumikiza peptide yotsatizana ya RGD ku utomoni. , ndi otsiriza amino asidi kuchotsa zoteteza gulu FMOC popanda piperidine.The chothandizira mwachindunji anawonjezera kuchotsa mbali unyolo carboxyl zoteteza gulu loyamba D aspartic asidi mwachindunji utomoni, kenako Kuwonjezera piperidine kuchotsa amino zoteteza gulu FMOC mapeto amino asidi, kenako Kuwonjezera kumanga wothandizila. kutsitsa madzi m'thupi ndi kusokoneza gulu la carboxyl ndi gulu la amino lowululidwa kuchokera kumutu ndi kumapeto kwa peptide yozungulira mwachindunji kuchokera ku utomoni mu mawonekedwe a amide bond kuti apange cyclic peptide.Pomaliza, cyclic peptide imadulidwa mwachindunji kuchokera ku utomoni ndi njira yodulira.