Kusintha kwa methylation

Ma peptides osinthidwa ndi methylation, omwe amadziwikanso kuti methylation-recognized peptides, ndi zokongoletsera zapambuyo pomasulira (PTMs) ndipo zimagwira ntchito yofunika kwambiri pakuwongolera pafupifupi zochitika zonse zamoyo m'maselo.Mapuloteni amapangidwa ndi methyltransferase kuti asamutse magulu a hydroxyl kupita ku zotsalira za amino acid kuti amangirire.Methylation ndi njira yosinthika yosinthika yopangidwa ndi demethylases.

Kafukufuku wasonyeza kuti wamba methylated/demethylated amino zidulo zambiri lysine (Lys) ndi arginine (Arg).Kafukufuku wapeza kuti histone lysine methylation ili ndi ntchito zosiyanasiyana zachilengedwe monga kukonza ma cell cell ndi kugawa, X chromosome inactivation, transcript regulation and DNA damage reaction.", nthawi zambiri imakhudza chromatin condensation ndipo imachepetsa jini."Histone arginine methylation imakhala ndi gawo lofunikira pakuwongolera kalembedwe ka jini ndipo imatha kukhudza machitidwe osiyanasiyana amthupi m'maselo, kuphatikiza kukonza kwa DNA, kutulutsa ma sign, kukula kwa cell, ndi carcinogenesis.Chifukwa chake, Guopeptide Biology yapanga mwapadera ukadaulo wa ma peptides okongoletsa a methyl, omwe amasinthidwa ndi asayansi pambuyo pomasulira mapuloteni (PTMS) kuti athandizire pa kafukufukuyu.

Kusintha kwa Methylation (Me1, Me2, Me3)

Fmoc-Lys(Me,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) 2-OH.HCl(asymmetrical), F idagwiritsidwa ntchito moc-Arg(me)2-OH.HCl(symmetrical) ndi zida zina zopangira zidapangidwa ndi njira yophatikizira ya FMOC kuti apeze Lys ndi Arg methylated peptides, ndi zinthuzo. adayeretsedwa ndi HPLC.Zowoneka bwino zofunikira, ma chromatogram a HPLC ndi COA amaperekedwa pazomaliza.