Kusintha kwa Methyty

Ma peptides osinthika, omwe amadziwikanso kuti ma peptides odziwika bwino, ndi zokongoletsera pambuyo pa protein (PTMS) ndikusewera gawo lolowera kwambiri pazinthu zonse zamoyo. Mapuloteni ndi othandizira ndi methyltransferase kuti asinthire magulu a hydroxyl kwa Amino Adid Yomwe Amakhala Kumandalama. Methylation ndi njira yosinthira yosinthika ndi demethyases.

Kafukufuku wawonetsa kuti metylated metylated / demethy yamino Acids nthawi zambiri imakhala lysine (LYS) ndi Arginine (arginine (arginine (arginine (arginine). Kafukufuku adapeza kuti rianine lysine methyletict ali ndi zochitika zachilengedwe monga stem cell. " Mbiri ya Arginine Itumbalalation imagwira gawo lofunikira pakuwongolera mtundu wa gener ndipo imatha kukhudza njira zosiyanasiyana zathupi m'maselo, kuphatikiza kwa cell, ndi carcinogeneis. Chifukwa chake, mafaloje sayansi ya Prooptide adapanga ukadaulo wa methyl chokongoletsera cha methyl, omwe amasinthidwa ndi asayansi pambuyo potanthauzira (PTM) kuti athandize pakufufuza.

Kusintha kwa Methylation (Me1, ME2, ME3)

FMOC yapamwamba kwambiri (ine, boc) -Oh, fmoc-lys (ineh, fmoc-arg (1) - fmoc-arg (asymmetrical), f adagwiritsidwa ntchito Moc-arg (ine) 2-oh.hcl (symmetricacal) ndi zina zopangira zina zimapangidwa ndi kaphatikizidwe kazinthu zopangira matsite kuti mupewe LYSYC yatsukidwa ndi HPLC. Misa Misa Spectra, hplc chromatogram ndi coa imaperekedwa kwa chomaliza.