Njira yatsopano yochitira ndi kukonzekera thymectomy

Njira yatsopano ya Thymus imapangidwa ndi 28 amino acid synthetic polypeptide immune regulator.Njira ya Thymus ndi mtundu wa mankhwala atsopano a uct, omwe amagwiritsidwa ntchito kwa odwala chotupa, amatha kuwonjezera chitetezo chokwanira.

Njira yochitira

Njira yeniyeni ya thymalfasin pochiza matenda a chiwindi a B sanaphunzire mokwanira, koma zoyesera zosiyanasiyana zasonyeza kuti thymalfasin ili ndi chitetezo champhamvu kwambiri.Zimatheka makamaka pakuwongolera magwiridwe antchito a T cell, ndipo imadutsa m'maiko omwe amaphatikiza kupanga ma T cell, kugawa ndi kukonzanso, ndi apoptosis.Poonjezera chiwerengero chokwanira cha lymphoid, intrahepatic natural killer, CD3 ndi CD4 cell, thymalfasin ikhoza kutenga mbali yoteteza chitetezo cha mthupi.Pleural fasin imatha kulimbikitsa kusasitsa ndi kugawanika kwa ma cell a DC, motero kumalimbikitsa kusasitsa ndi kugawa kwa ma T cell.Thymalfasin imatha kuyambitsa mitogen kapena antigen, kukulitsa kupanga kwamitundu yosiyanasiyana ya ma cytokines monga interferon human interleukin IL2 ndi IL3, kupititsa patsogolo mawu a interleukin-2 cholandilira, ndikupanga ma CD4T kusiyanitsa kukhala THLS, omwe ali ndi mphamvu zoletsa ma virus.Powonjezera gawo la P cell, imatha kulimbikitsa kupanga ma lymphocyte mu vivo, kusintha gawo la P cell subsets ndi U cell differentiation, komanso kupanga ma antibodies, kulimbikitsa kupanga ma antiviral lymphokines ndi macrophages, kukulitsa kumeza. ntchito, ndikuwongolera magwiridwe antchito a chitetezo chamthupi mwa odwala khansa.Ikhozanso kuchotsa mabakiteriya, mabakiteriya ndi mavairasi m'thupi.Buku la Thymus la dongosolo lamanjenje ndi endothelium yozungulira, amawuka kuti asinthe gawo la hippocampal neuron synaptic transmission.Thymus njira yatsopano yosangalatsa ya synaptic kufala ndi dongosolo lamanjenje, zitha kugwiritsidwa ntchito popewa odwala khansa omwe amayamba chifukwa cha chithandizo cha khansa monga chemotherapy ndi neurotoxicity.Pleuraxin ili ndi zotsatira zoyendetsera ma jini, imatha kuyambitsa majini a kinase, kuonjezera majini a cytokine, majini oyendetsa chitetezo cha mthupi.Kuwongolera kwa thymalfasin pamtundu wa IL-like receptor kukuwonetsa kuti thymalfasin imathandizira ma cell owonetsa ma antigen."Kuphatikiza apo, zotsatira za thymalfasin pakukula kwa maselo a chitetezo chamthupi zimawonekera pakuwonjezeka kwazinthu zokulirapo za maselo a stem komanso ma gene a hematopoietic protein."

Kutsatira kwatsopano kwa thymectomy

Njira yokonzekera

1. Njira yochotsera biochemical Kukonzekera kwa Thymosin (kusakaniza kwa peptides) kumachokera ku minofu ya nyama, motero ntchitoyo ndi yosiyana kwambiri.Chifukwa ili kale ndi mapuloteni a nyama, pali mwayi waukulu wa zizindikiro za ziwengo.The biochemically anapezedwa njira thymus kwa munthu embryonic thymus ndi latsopano, ndi zokolola zochepa kwambiri koma lachangu kwambiri.Poganizira zochepa zopangira, mtengo wapamwamba, zokolola zochepa, ndi njira zopezera zovuta., zovuta zopanga zazikulu, chiyembekezo chachitukuko sichikhala ndi chiyembekezo.

2. Kaphatikizidwe ka mankhwala Thymusfasin yomwe ikupezeka pamalonda imapangidwa makamaka ngati SPSS, yomwe imapangidwa ndikuyeretsedwa ndi njira zoyenera.Kutengera utomoni, Fmoc idagwiritsidwa ntchito popanga gawo lolimba, ndipo mitundu 28 ya ma amino acid idapangidwa molingana ndi thymalfasin.N-terminal amino acids anali acetylated ndi acetic anhydride, ndipo TFA idagwiritsidwa ntchito ngati chinthu chofunikira chophwanyira chophwanyira kuti tipeze zinthu zankhanza.Zogulitsazo zidalekanitsidwa ndi HPLC kuti ipeze njira yatsopano ya thymus, yomwe idayeretsedwanso kuti ipeze njira yatsopano ya thymus.

Pali mitundu itatu ya mabakiteriya opangidwa ndi majini1) Ma jini angapo amapangidwa kuti apeze ma jini omwe akufuna, ndipo pamapeto pake amawonetsedwa ngati matupi ophatikizika.Zanenedwa kuti mitundu yamtundu wamtundu wa thymmethod idakhazikitsidwa mwachisawawa komanso njira za PCR ndikusinthidwanso mu Escherichia coli.(2) Jini lopangidwa ndi mapuloteni ophatikizika adalumikizidwanso m'ma TV, ndipo zoulutsira nkhani zidasinthidwa kukhala omvera kuti apange mabakiteriya opangidwa ndi majini.Mwachitsanzo, kutengera jini ya peptide mu plasmid, mawu akuti plasmid adapangidwa, kusinthidwa kukhala Pichia pastoris ndi electroexcitation, ndiyeno mapuloteni ophatikizika adapangidwa ndi methanol, kenako njira yatsopano ya thymus idapangidwa ndi digestion ya enzyme ndi njira zina. .