Kufufuza kwachilengedwe, ma polypeptions ndi mikhalidwe yayitali nthawi zambiri amagwiritsidwa ntchito. Pa ma peptides okhala ndi ma acid oposa 60 amino acid omwe akhazikika, ma gene ndi tsamba la SDS nthawi zambiri amagwiritsidwa ntchito kuwapeza. Komabe, njirayi imatenga nthawi yayitali ndipo kulekanitsa kotsiriza sikwabwino.
Zovuta ndi Mayankho a Peptide Synthesis
Mu kapangidwe ka Peptides yotalika, timakhala tikukumana ndi vuto, ndiko kuti, kulephereka, kulepheretsa chosasangalatsa cha kuphatikizika kwa magawowo mu kaphatikizidwe, ndipo nthawi yochita iyenera kusinthidwa kuti izi zitheke. Komabe, nthawi yayitalinso, nthawi yovuta kwambiri imapangidwa, ndipo gawo la peptide yandamayo limapangidwa. Mafumu otere - kuchepa utatu zokwanira ndi zosafunikira zazikulu zomwe zimapangidwa mu kaphatikizidwe kambiri. Chifukwa chake, mu kaphatikizidwe ka peptide yayitali, vuto lalikulu lomwe tiyenera kugonjetsera ndikufufuza momwe zinthu zilili zapamwamba zomwe zimatengera machitidwe, kuti apangitse amino acid kukwaniritsidwa kwathunthu. Kuphatikiza apo, kuchepetsa nthawi yowonjezera, chifukwa nthawi yayitali yochita, mbali yosasunthika imachitika, zovuta kwambiri zopangidwa ndi zinthuzo. Chifukwa chake, mfundo zitatu zotsatirazi ndizofotokozedwa:
Ma Microwave Synthesis angagwiritsidwe ntchito: Kwa amino acid omwe adakumana nawo mu kapangidwe kake komwe sikophweka kuphatikizidwa, ma microwave synthesis angagwiritsidwe ntchito. Njirayi ili ndi zotsatira zabwino, ndipo zimachepetsa nthawi yochita, ndikuchepetsa mapangidwe awiri ofunikira.
Njira ya Synthesis ikhoza kugwiritsidwa ntchito: Ma Peptides ena akakhala ovuta kuphatikizidwa ndi njira zomwe zimachitika komanso zosavuta kuti tiyeretsedwe, titha kulolera kuvomerezedwa kwathunthu kwa zitsulo mbali inayake ya peputiyo kupita kuchipinda chonse. Njirayi imathetsanso mavuto ambiri mu synthesis.
Kaphatikizidwe ka acylhydrazide kungagwiritsidwe ntchito: Acylhyhydrazide synthesis ndi njira yophatikizira cyssis wa peptide ndi C-termide Polypeptide mankhwala kuti akwaniritse njira zomangira zomangira. Kutengera mawonekedwe a Cys mu Trepdide Tchere, njirayi imagawa utoto wonse wa pepipside kuti nawonso azolowere. Pomaliza, peputiyi imapezeka kudzera munthawi yochepa. Njira iyi siyochepetsa kungophatikizira kaphatikizidwe ka peptides yayitali, komanso zimawonjezera chiyero cha chinthu chomaliza.
Kudziyeretsa Kwakutali
Kutengera kwa ma peptides otalika kumabweretsa zovuta zovuta za ma peptides onyansa. Chifukwa chake, zimakhalanso zovuta kuyeretsa ma peplc atali ndi HPLC. Milandu ya Amyloiid a pulani yoyeretsa ma polytetide, ndikugwiritsa ntchito zambiri ndikugwiritsa ntchito bwino pakuyeretsa kwa itride. Mwa kukhala ndi zida zatsopano, kusakaniza makonzedwe angapo oyeretsa, kudzipatula mobwerezabwereza ndi njira zina zokumana nazo, kuchita bwino pakuyeretsa kwa nthawi yayitali kunayamba bwino.
Post Nthawi: 2025-07-03