Mavuto ndi mayankho a kaphatikizidwe ka peptide yayitali

Pakufufuza kwachilengedwe, ma polypeptides okhala ndi nthawi yayitali amagwiritsidwa ntchito.Kwa ma peptides okhala ndi ma amino acid opitilira 60 motsatizana, mawu a jini ndi SDS-PAGE nthawi zambiri amagwiritsidwa ntchito kuti awapeze.Komabe, njirayi imatenga nthawi yayitali ndipo zotsatira zolekanitsa zomaliza sizili zabwino.

Mavuto ndi mayankho a kaphatikizidwe ka peptide yayitali

Mu kaphatikizidwe ka ma peptides aatali, nthawi zonse timakumana ndi vuto, ndiye kuti, cholepheretsa chotchinga cha condensation chimawonjezeka ndi kuchuluka kwa kutsatizana mu kaphatikizidwe, ndipo nthawi yochitira imayenera kusinthidwa kuti zomwe zimachitikazo zitheke.Komabe, nthawi yayitali yochitapo kanthu, zotsatira zake zambiri zimapangidwa, ndipo gawo la peptide yomwe chandamale imapangidwa.Zotsalira zotere - maunyolo osowekera a peptide ndi zonyansa zazikulu zomwe zimapangidwa mu kaphatikizidwe ka peptide yayitali.Chifukwa chake, pakuphatikizika kwa peptide yayitali, vuto lalikulu lomwe tiyenera kuthana nalo ndikuwunika momwe zinthu ziliri komanso njira zomwe zimachitikira, kuti amino acid condensation reaction ikhale yokwanira komanso yodzaza.Kuonjezera apo, kuchepetsa nthawi yochitira, chifukwa nthawi yayitali kwambiri, momwe zimakhalira zosalamulirika, zomwe zimakhala zovuta kwambiri.Choncho, mfundo zitatu zotsatirazi zikufupikitsidwa:

Microwave synthesis ingagwiritsidwe ntchito: Kwa ma amino acid ena omwe amakumana nawo mu kaphatikizidwe kamene sikamakhala kosavuta kuphatikizidwa, kaphatikizidwe ka ma microwave angagwiritsidwe ntchito.Njirayi ili ndi zotsatira zochititsa chidwi, ndipo imachepetsa kwambiri nthawi yochitira, ndipo imachepetsa mapangidwe azinthu ziwiri zofunika kwambiri.

Fragment synthesis njira ingagwiritsidwe ntchito: Pamene ma peptide ena ali ovuta kupangidwa ndi njira zophatikizira wamba komanso zovuta kuyeretsedwa, titha kutengera kukhazikika kwa ma amino acid angapo mugawo lina la peptide kupita ku unyolo wonse wa peptide.Njirayi imathanso kuthetsa mavuto ambiri mu kaphatikizidwe.

Acylhydrazide synthesis angagwiritsidwe ntchito: acylhydrazide synthesis ya peptides ndi njira yolimba-gawo kaphatikizidwe wa N-terminal Cys peptide ndi C-terminal polypeptide hydrazide mankhwala kusankha zochita pakati pa mapangidwe amide zomangira kukwaniritsa peptide chomangira njira.Kutengera malo a Cys mu unyolo wa peptide, njirayi imagawaniza unyolo wonse wa peptide m'njira zingapo ndikuzipanga motsatana.Pomaliza, chandamale peptide imapezeka kudzera mumadzi-gawo condensation reaction.Njirayi sikuti imachepetsa kwambiri nthawi ya kaphatikizidwe ya peptide yayitali, komanso imawonjezera chiyero cha mankhwala omaliza.

Kuyeretsa kwa peptide yayitali

Kukhazikika kwa ma peptides aatali mosapeŵeka kumabweretsa zigawo zovuta za peptides zopanda pake.Chifukwa chake, ndizovuta kuyeretsa ma peptides aatali ndi HPLC.amyloid mndandanda wa polypeptide kuyeretsedwa ndondomeko, kuyamwa zambiri ndi bwino ntchito kuyeretsa peptide yaitali.Pogwiritsa ntchito zida zatsopano, kusakaniza machitidwe ambiri oyeretsera, kulekanitsa mobwerezabwereza ndi njira zina zodziwira, kupambana kwa kuyeretsa kwa peptide kwautali kunakula kwambiri.