Ma antigen ophatikizananso ndi mapuloteni nthawi zambiri amakhala ndi ma epitopes osiyanasiyana, ena mwa ma epitopes otsatizana ndipo ena ndi ma epitopes okhazikika.Ma antibodies a polyclonal omwe amapezedwa pobaya nyama zokhala ndi ma antigen opangidwa ndi denatured ndi osakanikirana a ma antibodies omwe ali ndi ma epitopes ndipo amatha kugwiritsidwa ntchito nthawi zonse kuti azindikire zachilengedwe kapena mapuloteni omwe amapangidwa.Ubwino wina wogwiritsa ntchito mapuloteni opangidwa ngati ma immunogens ndikuti mapuloteni opangidwa ndi denatured amakhala ochulukirapo ndipo amatha kulimbikitsa chitetezo chamthupi mwa nyama.
Kachitidwe ka mawu a Escherichia coli kaŵirikaŵiri amasankhidwira zolinga za antigenic chifukwa ndi dongosolo lokwera mtengo kwambiri potengera nthawi ndi ndalama.Pofuna kupititsa patsogolo kuthekera kwa kufotokoza kwa mapuloteni omwe akuwunikira komanso kuphweka kwa kuyeretsedwa, nthawi zina kachigawo kakang'ono ka mapuloteni omwe akufuna, monga malo enieni, amawonetsedwa.
Mapangidwe amitundu itatu ya mapuloteni
Domain Yeniyeni Ya Mapuloteni
Ngati cholinga chokonzekera ma antibody ndikungodziwidwa ndi wb, ndizochuma komanso zofulumira kugwiritsa ntchito peptide yaying'ono yopanga ngati antigen, koma pali chiwopsezo cha kufooka kwa chitetezo chamthupi kapena kusabadwanso chifukwa cha kusankha kosayenera kwa gawo la peptide.Popeza kukonzekera kwa antibody kumafuna nthawi yayitali, magawo awiri kapena atatu osiyana a peptide nthawi zambiri amasankhidwa kuti akonzekere ma antibodies pogwiritsa ntchito polypeptide antigen kuti awonetsetse kuti kuyesako kukuyenda bwino.
Kuyera kwa antigen ya polypeptide pakutemera kumafunika kukhala wamkulu kuposa 80%.Ngakhale kuti chiyero chapamwamba chikhoza kupeza ma antibodies omwe ali ndi chidziwitso chabwinoko, pochita, nyama nthawi zonse zimatulutsa ma antibodies ambiri omwe si enieni, motero amabisa ubwino wa antigen chiyero.
Kuphatikiza apo, kukonzekera kwa ma antibodies ochokera ku peptides ang'onoang'ono kuyenera kulumikizidwa ndi antigen yoyenera chonyamulira kuti apititse patsogolo chitetezo chake.Zonyamula ziwiri zodziwika bwino za antigenic ndi KLH ndi BSA.
Nthawi yotumiza: Mar-23-2023