Amakonda kwambiri ku HPLC kulephera ndi mayankho

Monga chida chowongolera kwambiri, HPLC imatha kutsogolera zovuta zazing'ono ngati sizikugwira ntchito molondola panthawi yogwiritsa ntchito. Chimodzi mwazovuta zambiri ndiye vuto la chiwonetserochi. Momwe mungasinthire mofulumira chromatography yolakwika. Dongosolo la HPLC limakhala ndi botolo losungika, pampu, wolowetsa, mzere, chipinda chotentha chotentha, cholembera ndi dongosolo la deta. Kwa dongosolo lonse, zipilala, mapampu ndi zowunikira ndiye zigawo zazikulu komanso malo akuluakulu omwe amakonda mavuto.

Chinsinsi cha kupanikizika ndi gawo lomwe limafunikira chisamaliro chapamtima mukamagwiritsa ntchito HPLC. Kukhazikika kwa kupanikizika kwa colum ndikugwirizana kwambiri ndi mawonekedwe a chromatographic mawonekedwe, cholembera chowongolera, chowongolera chodzipatula komanso nthawi yosunga. Kukhazikika kwa mtunduwo sikutanthauza kuti mtengo wokakamizidwa ndi wokhazikika pamtengo wokhazikika, koma makamaka kuti mitundu yosinthira kupanikizika ili pakati pa 345kPa kapena 50kpa kapena 50kpu kapena 50psi (kulola kugwiritsa ntchito mawonekedwe okhazikika pomwe panali kusintha pang'onopang'ono). Okwera kwambiri kapena otsika kwambiri ndi vuto la kupindika.

Amakonda kwambiri ku HPLC kulephera ndi mayankho

1, kupanikizika kwambiri ndiye vuto lodziwika bwino pakugwiritsa ntchito HPLC. Izi zikutanthauza kukwera mwadzidzidzi. Mwambiri, pali zifukwa zotsatirazi: (1) Mwambiri, izi zimachitika poyambira kuyenda. Pakadali pano, tiyenera kuzifufuza zandale. a. Choyamba, dulani cholowa cha pampu ya vacuum. Pakadali pano, chubu cha peek chinadzaza ndi madzi kuti balu likhale laling'ono kwambiri kuposa botolo la solnt kuti muwone ngati madziwo akuwuma. Ngati madziwo sakukhetsa kapena kudzutsa pang'onopang'ono, mutu wa suphntn watsekedwa. Chithandizo: zilowerere mu 30% nitric acid kwa theka la ola ndikutsuka ndi madzi okwanira. Ngati madzi akudulidwa mwachisawawa, mutu wa suphntn ndi wabwinobwino ndipo ukuyang'aniridwa; b. Tsegulani valavu yoyeretsa kuti gawo lam'manja silidutsa mzati, ndipo ngati kukakamizidwa sikunachepe mwamphamvu, mutu wakuyera watsekedwa. Chithandizo: Ma whitead achotsedwa ndi kunenedwa ndi 10% isopropanol kwa theka la ola. Kungoganiza kuti kupanikizika kumatsika pansi pa 100psi, mutu wofota ndi wabwinobwino ndipo akuyang'aniridwa; c. Chotsani kumapeto kwa mzati, ngati kupsinjika sikuchepera, mzatiyo watsekedwa. Chithandizo: Ngati ndi malo amchere amchere, mutsetsere 95% mpaka kupsinjika kuli kwachilendo. Ngati chotsekanitsidwa ndi chinthu china chosungidwa kwambiri, choyenda champhamvu kuposa gawo la foni lino liyenera kugwiritsidwa ntchito pothamanga. Ngati kupanikizika kwa nthawi yayitali sikuchepa malinga ndi njira yomwe ili pamwambapa, yotsekera ndi malo otulutsa mzati yomwe ingaonedwe kuti ilumikizidwe ndi chida chosiyana, ndipo mzerewu ungathe kutsukidwa ndi gawo lam'manja. Pakadali pano, ngati kulumikizana kwa nambala sikunathebe, mbale yopaka kwa chipapuno imatha kusinthidwa, koma opareshoni siabwino, ndikosavuta kuchepetsedwa ndi cholembera, choncho yesani kugwiritsa ntchito zochepa. Pamavuto ovuta, kuphatikizika kwa mzati kungaganizidwe.

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. Ngati ndi kotheka, otsetsereka a ViscCon Asccent amatha kusinthidwa kapena kukhazikitsidwanso ndikukonzekera.

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2, kukakamizidwa kuli kotsika kwambiri (1) nthawi zambiri kumachitika chifukwa cha kuyika kwa dongosolo. Zoyenera Kuchita: Pezani kulumikizana kulikonse, makamaka mawonekedwe kumapeto onsewa, ndikulimbana ndi malo. Chotsani positi ndikulimba kapena mzere wa PTF ndi mphamvu yoyenera.

(2) Mafuta amalowa pampu, koma kupsinjika nthawi zambiri kumakhala kosakhazikika pakadali pano, kutalika komanso kotsika. Mozama, pampu satha kuyamwa madzimadzi. Njira Yothandizira: Tsegulani valavu yoyeretsa ndikuyeretsa pa 3 ~ 5ml / mphindi. Ngati sichoncho, thovu la mpweya linasiyidwa pa valavu yotulutsa pogwiritsa ntchito chubu chodzipereka.

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. Nthawi zambiri zimatsika mpaka 0.1. ~ 0.2ml / mphindi mutatseka valavu.

Chidule:

Mu pepala ili, mavuto wamba omwe amadzimadzi a chromatography amasanthuridwa. Inde, pogwiritsa ntchito kwathu, tidzakumananso ndi mavuto enanso. Pogwiritsira ntchito molakwika, tiyenera kutsatira mfundo zotsatirazi: Ingosinthani chinthu chimodzi panthawi kuti tidziwe ubale womwe ulipo pakati pa vutoli; Nthawi zambiri, posinthanso magawo osokoneza, tiyenera kulabadira kuyika magawo osokonekera m'malo kuti mupewe zinyalala; Kupanga chizolowezi chabwino cholembedwa ndi chinsinsi cha kupambana kwa kuthana ndi vuto. Pomaliza, mukamagwiritsa ntchito HPLC, ndikofunikira kumvetsera mwachidwi komanso kugwira ntchito moyenera ndi kukonza zida.