Monga chida cholondola kwambiri, HPLC imatha kuyambitsa mavuto ang'onoang'ono ovuta ngati sichigwiritsidwa ntchito moyenera panthawi yogwiritsidwa ntchito.Limodzi mwamavuto omwe amafala kwambiri ndi vuto la kupsinjika ndime.Momwe mungathetsere mwachangu chromatograph yolakwika.Dongosolo la HPLC makamaka limapangidwa ndi botolo la posungira, pampu, jekeseni, ndime, chipinda cha kutentha, chowunikira ndi makina opangira deta.Kwa dongosolo lonse, zipilala, mapampu ndi zowunikira ndizo zigawo zikuluzikulu ndi malo akuluakulu omwe amakhala ovuta kwambiri.
Chinsinsi cha kupanikizika kwa mizere ndi malo omwe amafunikira chidwi kwambiri mukamagwiritsa ntchito HPLC.Kukhazikika kwa kupanikizika kwa mizere kumagwirizana kwambiri ndi mawonekedwe apamwamba a chromatographic, magwiridwe antchito amzanja, kupatukana bwino komanso nthawi yosunga.Kukhazikika kwapakati pazigawo sizikutanthauza kuti kupanikizika kwapakati kumakhala kokhazikika pamtengo wokhazikika, koma kuti kusinthasintha kwapakati pa 345kPa kapena 50PSI (kulola kugwiritsa ntchito gradient elution pamene kupanikizika kwa mzere kumakhala kokhazikika komanso kusintha pang'onopang'ono).Kuthamanga kwambiri kapena kutsika kwambiri ndi vuto lazambiri.
Zovuta kwambiri za HPLC zolephera ndi zothetsera
1, kuthamanga kwambiri ndiye vuto lofala kwambiri pakugwiritsa ntchito HPLC.Izi zikutanthauza kukwera kwadzidzidzi kwa kukakamizidwa.Nthawi zambiri, pali zifukwa zotsatirazi: (1) Nthawi zambiri, izi zimachitika chifukwa cha kutsekeka kwa njira.Panthawi imeneyi, tiyenera kuganizira pang'ono.a.Choyamba, dulani cholowera cha pampu vacuum.Panthawiyi, chubu cha PEEK chinadzazidwa ndi madzi kotero kuti chubu la PEEK linali laling'ono kuposa botolo losungunulira kuti liwone ngati madzi akudontha mwakufuna kwake.Ngati madziwo sakudontha kapena kudontha pang'onopang'ono, mutu wa zosungunulira umatsekedwa.Chithandizo: Zilowerereni mu 30% nitric acid kwa theka la ola ndikutsuka ndi madzi owonjezera.Ngati madzi akudontha mwachisawawa, mutu wa zosungunulira zosungunulira ndi wabwinobwino ndipo ukufufuzidwa;b.Tsegulani valavu ya Purge kuti gawo la mafoni lisadutse pamtanda, ndipo ngati kupanikizika sikuchepetsedwa kwambiri, mutu woyera wa fyuluta umatsekedwa.Chithandizo: Zosefera whiteheads anachotsedwa ndi sonicated ndi 10% isopropanol kwa theka la ola.Poganiza kuti kupanikizika kumatsika pansi pa 100PSI, mutu woyera wosefedwa ndi wabwinobwino ndipo ukufufuzidwa;c.Chotsani kumapeto kwa gawolo, ngati kupanikizika sikucheperachepera, gawolo limatsekedwa.Chithandizo: Ngati ndi chotchinga mchere chotchinga, muzimutsuka 95% mpaka kupanikizika kuli bwino.Ngati chotchingacho chachitika chifukwa cha zinthu zina zotetezedwa kwambiri, kuthamanga kwamphamvu kuposa gawo lomwe lilipo kuyenera kugwiritsidwa ntchito kuthamangira kukakamiza kwanthawi zonse.Ngati kupanikizika kwa nthawi yayitali sikucheperachepera malinga ndi njira yomwe ili pamwambayi, kulowetsa ndi kutuluka kwa gawoli kungaganizidwe kuti kukugwirizana ndi chida m'malo mwake, ndipo gawoli likhoza kutsukidwa ndi gawo la mafoni.Panthawiyi, ngati kupanikizika kwa mzati sikunachepetsebe, mbale yolowera sieve ingasinthidwe kokha, koma pamene ntchitoyo si yabwino, n'zosavuta kutsogolera kuchepetsa zotsatira za mzati, choncho yesetsani kugwiritsa ntchito zochepa.Kwa zovuta zovuta, kusintha kwa magawo kungaganizidwe.
(2) Kuyika kwa mlingo wolakwika: Kuthamanga kolondola kungathe kukonzanso.
(3) Chiŵerengero cha otaya molakwika: index mamasukidwe akayendedwe osiyanasiyana otuluka ndi osiyana, ndi lolingana dongosolo kuthamanga kwa otaya ndi kukhuthala kwapamwamba kumakhalanso yaikulu.Ngati n'kotheka, zosungunulira za viscosity zochepa zimatha kusinthidwa kapena kukhazikitsidwanso ndikukonzedwa.
(4) Kuthamanga kwa dongosolo zero: sinthani zero ya sensor yamadzimadzi.
2, kupanikizika kumakhala kochepa kwambiri (1) nthawi zambiri kumachitika chifukwa cha kutayikira kwadongosolo.Zoyenera kuchita: Pezani kulumikizana kulikonse, makamaka mawonekedwe omwe ali kumapeto kwa gawoli, ndikumangitsa malo otayira.Chotsani positi ndikumangitsa kapena kulumikiza filimu ya PTFE ndi mphamvu yoyenera.
(2) Mpweya umalowa mu mpope, koma kupanikizika nthawi zambiri kumakhala kosakhazikika panthawiyi, kutsika komanso kutsika.Zowonjezereka, mpope sudzatha kuyamwa madziwa.Njira yothandizira: tsegulani valavu yoyeretsera ndikuyeretsa pamtunda wa 3 ~ 5ml / min.Ngati sichoncho, tinthu tating'onoting'ono ta mpweya timapukutira pa valve yotulutsa mpweya pogwiritsa ntchito chubu cha singano.
(3) Palibe kutuluka kwa gawo la mafoni: fufuzani ngati pali gawo la mafoni mu botolo losungiramo madzi, ngati sink imamizidwa mu gawo la mafoni, komanso ngati mpope ikuyenda.
(4) Valve yolozerayo sinatsekedwe: valavu yolumikizira imatsekedwa pambuyo pakutsika.Nthawi zambiri zimatsikira ku 0.1.~ 0.2mL/ min mutatha kutseka valve yofotokozera.
Chidule:
Papepalali, mavuto omwe amapezeka mu chromatography yamadzimadzi amawunikidwa.Ndithudi, m’kugwiritsira ntchito kwathu kothandiza, tidzakumana ndi mavuto ena owonjezereka.Pochita zolakwika, tiyenera kutsatira mfundo zotsatirazi: kungosintha chinthu chimodzi panthawi imodzi kuti tidziwe mgwirizano pakati pa chinthu chongoganizira ndi vuto;Nthawi zambiri, posintha magawo kuti athetse mavuto, tiyenera kulabadira kubweza mbali zomwe zidaphwasulidwa m'malo mwake kuti tipewe zinyalala;Kupanga chizolowezi cholemba bwino ndiye chinsinsi cha kupambana kwa kuwongolera zolakwika.Pomaliza, mukamagwiritsa ntchito HPLC, ndikofunikira kulabadira zitsanzo za pretreatment ndikugwiritsa ntchito moyenera ndikukonza zida.
Nthawi yotumiza: Sep-18-2023