Imakhala ndi gawo lofunikira pamoyo, mtundu wa phosylang, ser, pampando, womwe umapezeka pama peptides. The phosphonet amino amagwiritsidwa ntchito popanga phosphoroc acid phukusi, lomwe limagwiritsidwa ntchito pa phenyl phossolted amino acid. HCTU / phossoryted amino acid amagwiritsidwa ntchito kulumikiza phosphonnated amino acid / dino Njira yofikira kwambiri, koma polumikiza ntchitoyo imakhala yotsika, kuyera kotsika mtengo. Chifukwa cha phosphopheptide iyi, takambirana njira yosankha positi. Njira yoyambitsa ndi kusankha kwa amino acid khoma-unyolo woteteza magulu omwe amafunika kulembedwa pambuyo pa Toptide Synthesis. Kwa wopusa, ngongole imatha kugwiritsa ntchito mwachindunji pogwiritsa ntchito ma amino acid ndi maunyolo osatetezeka. -Kuchotsa (TRT), kuchuluka kwa 1% ya TFA / DCM.
Phosserary, wokhala ndi benzene phosphramide ndi nayitrogeni amapangidwa ndipo phspheramide tetrazolium yogwira, kenako pansi pa acid peroxide oxidation, atero.
Pakadali pano, njira yosinthira phosthory phoshonevey pali mitundu iwiri yayikulu:
.
.
1) Kodi modeaffic chitsimikizo cha phossolt amino acid mwachindunji amayambitsa mwachindunji peptide motsatizana:
Ndiye kuti, amino acid omwe akufunika kukhala phosphorylated idzakhala yotetezedwa mosamala kugwiritsa ntchito (kwa kaphongo kapena Tyr), kenako therr kapena Tyr), kenako therr kapena Tyr), kenako Njirayi ndiyosavuta kuyigwiritsa ntchito, yakhala njira yayikulu ya Puptide Bizinesi ya Phosphoryted kusintha.
2) Pambuyo pa utoto womwe umapangidwa pamndandanda wa peptide, ku Serroxy phossorylation, tyr, kapena maunyolo mbali mbali:
Kukongoletsa phosyry M'tsogolo, makonzedwe a amino acid adzakhala ovuta kwambiri, makamaka pazokongoletsera zambiri zaphoto, mbadwo womaliza uzikhala wovuta, wokolola ndi wotsika kwambiri.
Chifukwa chake, pamene malo angapo mu peptide aja ndi phossovena, munthu amatha kusankha kupanga chofufumitsa pa slin ndipo kenako phosphorylate ndi gulu la ma hydroxyl acid pambuyo pa Peelnide. Kwa wa tyr, pampunga zimatha kugwiritsa ntchito mwachindunji amino acid mbali siyitetezedwe.
Chitetezo cha MITUNGU 1% TFA / DCM Crea amatha kuchotsedwa. Njirayi ikasankhidwa, phsphomididi phoskazolium yogwira ntchito yapakati imatha kugwiritsidwa ntchito pa dibenzonium buspomiti ndi gulu la hydrobyl, kenako ndikupanga gulu la phosphoryl kuti likwaniritse zomwe zachitikazo.
Post Nthawi: 2025-07-02