Mankhwala opezekapo nthawi zambiri amadziwika kuti ma poider adapangidwa ndi ma amder ochepera 40 amino admies ochepera 40 amino acid. Chifukwa cha ntchito yolandila ndi kusankhidwa kwa peptide mankhwala omwe ali pachiwopsezo cha zovuta, pakhala pali chidwi ndi ma peptides ochokera m'makampani opanga mankhwala. Munthawi imeneyi, panali mankhwala osokoneza bongo ambiri, omwe anali otanganidwa kwambiri ndi makampani a metabolic, monga Glp-1 Soldiory Peptidide (Gip) Kuphatikiza apo, ndikukwera kwa mankhwala osokoneza bongo a PDC ndi RDC. Pakadali pano, njira zokonzekera za polypeption mankhwala zimaphatikizapo ma dentisis a mankhwala ndi kugwedeza kwachilengedwe. Biofermentation is mainly used to produce long peptides. The advantages are low production costs, but the inability to introduce unnatural amino acids into the peptide sequence and the inability to perform various decorations on the peptide chain. Chifukwa chake, ntchito yake ilinso yochepa. Njira zamankhwala njira zimaphatikizira gawo lolimba la gawo la magawo ndi gawo lamadzimadzi. Kaphatikizidwe kakang'ono ka gawo kamakhala ndi mwayi wofunika kwambiri kuposa kaphatikizidwe ka gawo: kuchuluka kwa zinthu kungagwiritsidwe ntchito pofuna kuonetsetsa kuti atsimikiza. Excess amino acids, shrinkage agents, and by-products can be removed by simple cleaning operations, avoiding complex post-processing and purification operations and improving work efficiency, so the solid-phase synthesis method has been the most widely used. "Mankhwala a Mankhwala Osiyanasiyana a kapangidwe ka ma peptis amaphatikiza zoyambira, ma khwangwala, ndi sol sol." Khalidwe lawo, makamaka mtundu wa zoyambira, zimatha kukhala ndi zovuta pa mtundu wa api. The starting material mainly refers to the guaranteed amino acid derivatives for peptide chain modified fatty acids, polyethylene glycol, etc. As important structural fragments, they are classified as materials in the API structure, which is directly related to the quality of the API. Chifukwa chake, tiyenera kuganizira kwambiri za kuwongolera kwa zinthu zoyambira.
I. Mwachidule kusankha koyamba
ILQ11 imalongosola bwino kuti ngati mankhwala ogulitsidwa pamsika amagwiritsidwa ntchito ngati zinthu zoyambirira zoyambirira, wofunsayo sayenera kukambirana chifukwa chake. Zogulitsa zamankhwala zomwe zimagulitsidwa pamsika nthawi zambiri sizingagwiritsidwe ntchito ngati zida zoyambira mankhwala, komanso zitha kugulitsidwa m'misika yopanda mankhwala. Makonda opangidwa ndi mapangidwe osakhala a zinthu za mankhwala ogulitsidwa pamsika. Ngakhale kuti palibe msika wamankhwala woteteza amino acid akukumana ndi matchulidwe a ICHQ11 omwe amagulitsidwa pamsika, ndi yaying'ono, yodziwika bwino komanso yodziwikiratu komanso yoyesedwa ndi njira wamba. Ali ndi zida zokhazikika ndipo ndizosavuta kusunga, kunyamula, ndi kuphatikizika
Ii. Kuwongolera zinthu zomwe zilipo poyambira
Amino otetezedwa ndi amino omwe tafotokozawa amaphatikizidwa mu kapangidwe ka API monga gawo lofunikira, lomwe likugwirizana mwachindunji ndi mtundu wa api. Chifukwa chake, tiyenera kupewa kudekha mwakudetsa m'zinthu zoyambirira, kumvetsetsa kusinthaku ndikuchotsa izi mwanjira yokhazikitsidwa, ndipo pamapeto pake fotokozerani ubale pakati pawo ndi zosayera mu API.
Kumvetsetsa kwa Matanda Ogulitsa Polypeptide
Chachitatu, zosungunulira m'zinthu zoyambirira
In general, given the specificity of solid phase generation of peptides, a large amount of solvent will be used to clean the peptide resin after completing each step of amino acid coupling and detachment from protection. Crude peptides obtained by cracking the peptide resin will also be made by HPLC and freeze-dried. Thus, there is little risk that the small amount of solvent attached to the protective amino acids will be delivered to the final API. However, special attention should be paid to residues of acetate, butyl acetate, and alcohol solvents, as these solvents may cause side effects with active amino acids or peptide chains during active coupling of amino acids. Mwachitsanzo, mu Amino Ading, otsalira acetic acid adzachita ndi gulu lowonekera la amino pa utoto wa peptide. During amino acid activity, the residual alcohol solvent may react with the active carboxyl group, leading to passivation of the active amino acid, reducing the equivalent of the amino acid, and ultimately resulting in incomplete amino acid coupling and lack of peptide impurities. The company controls butyl acetate, alcohol, methanol, and acetic acid in COA, taking an amino acid from Zheng Yuan Biochemical as an example. Muyeso wa ndyl acetate anali ≤0.5% andyl acetate, omwe adazindikirika kukhala 0.10%. Malinga ndi ichq3c, komatyl acetate kwa mitundu itatu ya ma sodi, ikani muyeso wa 0,5% kapena zochepa mogwirizana ndi zofuna za Icetylate amino kuti mukwaniritse kafukufuku, kuti adziwe zofufuzira, kuti mudziwe mfundo zoyenera.
Post Nthawi: 2025-07-02