Ma peptide olowera m'maselo ndi ma peptides ang'onoang'ono omwe amatha kulowa mosavuta mu cell membrane.Gulu la mamolekyu, makamaka ma CPP omwe ali ndi ntchito zolunjika, amakhala ndi lonjezo lopereka mankhwala oyenera kumaselo omwe akutsata.
Chifukwa chake, kafukufuku wokhudza izi ali ndi tanthauzo lina lazachilengedwe.Mu phunziro ili, ma CPP omwe ali ndi ntchito zosiyanasiyana za transmembrane anaphunziridwa pamlingo wotsatizana, kuyesera kupeza zinthu zomwe zimakhudza ntchito ya transmembrane ya CPPs, kusiyana kwa ma CPP omwe ali ndi ntchito zosiyanasiyana ndi NonCPPs, ndikuyambitsa njira yowunikira machitidwe achilengedwe.
Ma CPP ndi ma NonCPP amatsatiridwa kuchokera ku database ya CPPsite ndi zolemba zosiyanasiyana, ndipo ma transmembrane peptides (HCPPs, MCPPs, LCPPs) okhala ndi ntchito zapamwamba, zapakatikati, ndi zotsika za transmembrane zinachotsedwa muzotsatira za CPPs kuti apange ma data.Kutengera ma data awa, maphunziro otsatirawa adachitika:
1, Amino acid ndi mawonekedwe achiwiri a CPPs yogwira ntchito ndi NonCPPs adawunikidwa ndi ANOVA.Zinapezeka kuti kuyanjana kwa electrostatic ndi hydrophobic kwa amino acid kunathandiza kwambiri pa ntchito ya transmembrane ya CPPs, ndipo mawonekedwe a helical ndi kupopera mwachisawawa kunakhudzanso ntchito ya transmembrane ya CPPs.
2. Zakuthupi ndi mankhwala ndi kutalika kwa ma CPP okhala ndi ntchito zosiyanasiyana adawonetsedwa pa ndege yamitundu iwiri.Zinapezeka kuti ma CPP ndi NonCPP omwe ali ndi ntchito zosiyanasiyana akhoza kuphatikizidwa pansi pa katundu wina wapadera, ndipo HCPP, MCPPs, LCPPs ndi NonCPPs adagawidwa m'magulu atatu, kusonyeza kusiyana kwawo;
3. Mu pepala ili, lingaliro la thupi ndi mankhwala centroid la zochitika zamoyo zimayambitsidwa, ndipo zotsalira zomwe zimapanga ndondomekoyi zimatengedwa ngati tinthu tating'onoting'ono, ndipo ndondomekoyi imatengedwa ngati tinthu tating'ono tofufuza.Njirayi idagwiritsidwa ntchito pakuwunika ma CPP powonetsa ma CPP okhala ndi zochitika zosiyanasiyana pa ndege ya 3D ndi njira ya PCA, ndipo zidapezeka kuti ma CPP ambiri amalumikizana pamodzi ndipo ma LCPP ena amalumikizana pamodzi ndi NonCPP.
Kafukufukuyu ali ndi zotsatira za mapangidwe a CPPs ndikumvetsetsa kusiyana kwa ma CPP omwe ali ndi ntchito zosiyanasiyana.Kuphatikiza apo, njira yowunikira ma centroid akuthupi ndi amankhwala amayendedwe achilengedwe omwe atulutsidwa mu pepalali angagwiritsidwenso ntchito powunika zovuta zina zamoyo.Nthawi yomweyo, atha kugwiritsidwa ntchito ngati magawo olowera pamavuto ena am'magulu achilengedwe ndikuthandizira kuzindikira mawonekedwe.
Nthawi yotumiza: Jun-15-2023