Makina obiriwira a Limulus omwe ali pachimake ndi chinthu choyambirira chomwe chimachokera ku Horvershoe Crab magazi ysate kapena zopangidwa mwamphamvu. Trulus Realement Tripept ndi gawo la chromogenic lomwe limagwiritsidwa ntchito popendaponda (monga bucteria test (monga ma bakiteriya—endotoxins. Mukakhala ndi ma enzymes oyambitsidwa ndi endotoxins, chophatikizika (monga p-nutroniline) amasulidwa, kulola kukomoka kwa utoto kuti uyesedwe ndikusinthana ndi zomwe zili ndi Endotoxn.
Mfundo
Kukhazikitsa kwa Endotoxin: Pamaso pa endotoxins, amebocytes mu kavalo wa hobocthoe crab magazi kutulutsa michere, potero kusokoneza ma coagcade.
Enzyme zochita: Enzyme yoyambitsidwa (nthawi zambiri enzyme) imachitika pa chromogenic gawo lapansi.
Chromogenic 2. Enzyme imatulutsa gawo lapansi, ndikumasula ma glake achikasu otchedwa P-Nitroniline (PNA).
Kusanthula Kwakukulu: Kukula kwa mtundu wachikasu ndikofanana ndi mawu a endotoxn mu zitsanzo ndipo nthawi zambiri kumayesedwa pogwiritsa ntchito mawonekedwe a 405 nm.
Kukonzekera Njira
Kuwala kwa lingulus kumatha kupezeka kudzera njira ziwiri pankhani yokonzekera.
1. Kutulutsa zinthu zachilengedwe kuchokera ku Limiulus maselo am'magazi ndikupeza chipongwe chogwira ntchito kudzera mu kupatukana ndi kuyeretsa.
2. Gwiritsani ntchito ukadaulo wolimba wokhazikika kuti mulumikizane ndi Amino Acids, kuwongolera kapangidwe ka Thipetopside.
Chifukwa cha njira zotetezera a Lilulus (ndi zigawo monga Zhejiang, Fujian, Guangdong, ndi Gung Kong Wongling Malamulo " Chifukwa chake, kapangidwe ka gawo kamasamba tsopano kwagwiritsidwa ntchito. Mwa kuphatikiza mwatsatanetsatane amino, ma acipe acid amatha kulamulidwa ndendende. Njira iyi siyingotsimikizira kukhazikika ndi kusasinthika kwa chinthucho komanso kumathandizanso kupanga kwakukulu, kukwaniritsa zosowa za malonda opangira zoyeserera.
Post Nthawi: 2025-11-04