Pal-AHK ikuwoneka ngati tripeptide yokhala ndi alanine-histidine-lysine, yomwe yapangidwa palmitoylated kudzera pa C16 fatty acid chain kupita ku N-terminus yotsatizana.(1)Kutsatizana kwa peptide kumawoneka kuti kumagwirizana kwambiri ndi ayoni amkuwa, pomwe palmitoylation ikuwoneka kuti imathandizira lipophilicity ya pawiri.
Pal-AHK peptide ndiAmaphunziridwa makamaka chifukwa cha kuyanjana kwake ndi ma dermal papilla cell (DPCs) omwe amayikidwa kuti akhale ngati maselo oyendetsera tsitsi. Peptide imatha kutero polumikizana ndi zinthu zosiyanasiyana zakukulira zomwe zimayendetsa kuchuluka kwa ma cell, apoptosis, ndi vascularization.
Pal-AHK Kapangidwe
In silico analysis by Kecel-Gunduza et al. akuwonetsa kuti kutsatizana kwa AHK kumatha kukhala kogwirizana kwambiri ndi ayoni amkuwa, omwe amatha kuyendetsedwa ndi zotsalira za histidine, zomwe unyolo wam'mbali wa imidazole umakhala ngati malo oyambira a Cu²⁺ ions kudzera mu chemistry yolumikizana.(2)Gulu la amino la Lysine lingaperekenso kukhazikika kowonjezereka, pamene alanine imathandizira kusinthasintha kwapangidwe.
Zotsalirazi zimayikidwa kuti zikhalebe bwino ngakhale pambuyo pa palmitoylation, pamene ndondomekoyi ikuwonjezera C16 fatty acid chain ku N-terminus ya peptide, makamaka zotsalira za alanine, kusiya malo ogwirizanitsa a histidine osasokonezeka. Njira ya palmitoylation imayikidwanso kuti ithandizire kuthekera kwa peptide kulowa kudzera m'mapangidwe a lipophilic monga zikhalidwe zama cell a dermal cell ndi ma labotale ofanana.
Pal-AHK Njira ndi Ma Cellular Apoptosis
Sadgrove et al. akuwonetsa kuti njira zazikulu zomwe zimapangitsa kuti peptide igwirizane ndi ma cell owongolera a tsitsi la mammalian zingaphatikizepo"Kuchepetsa kufotokozera kwa TGF-β1 ndi kuwonjezeka kwa shaft ya tsitsi, kuwonjezereka kwa mitsempha ya endothelial kukula factor ndi kuchepetsa kukula kwa zinthu zoipa."(3)Kuphatikiza apo, ofufuzawa amatchula pepala la Pyo et al. zomwe zimasonyeza kuti pamene maselo a follicular epithelial amawonekera ku peptide, omalizawa amatha kulimbikitsa kufalikira ndi kusiyanitsa.
Kumanga Pal-AHK ku ayoni amkuwa kumatha kuthandizira izi.(4)Pepalalo likuwonetsa kuti peptide ikhoza kukhala ndi anti-apoptotic action pama cell a dermal papilla, kuchepetsa apoptosis ndi pafupifupi 3.48%. Kuchepetsa pang'onopang'ono kwa ma cell a apoptotic kunathandizidwanso pamlingo wa mapuloteni, pomwe peptide idawoneka kuti ikusintha malire pakati pa olamulira a pro- ndi anti-apoptotic mkati mwa banja la Bcl-2, kutanthauza kuti zochitazo zitha kupangidwanso mwamakina ngakhale sizinali zolimba pamlingo wowerengera maselo.
Popeza kuti mawu a Bcl-2 amanenedwa kukhala otsogola pakusintha kwa telogen-to-anagen, kusinthaku kungakhale kogwirizana ndi kulowanso kwa ma cell atsitsi. Mogwirizana ndi kusintha kwa Bcl-2/Bax, protease wina wapakati pa apoptotic cascades wotchedwa Caspase-3 angayankhenso ku Pal-AHK, makamaka pamene chotsatiracho chimamangiriza ku ayoni amkuwa. Pyo ndi al. akuwonetsa kuti peptide ikhoza kuchepetsa mawonekedwe a caspase-3 pafupifupi 42.7% ndi chimodzi mwazofunikira zake zoyambira pansi, zomwe zimatchedwa PARP, pafupifupi 77.5%, pomwe ma procaspase-3 mwachiwonekere sanasinthe.
Kafukufukuyu adawonetsanso kuti chifukwa cha kufanana kwapangidwe pakati pa machitidwe a AHK ndi GHK, Pal-AHK akhoza kuyanjananso ndi zinthu za kukula, monga kukweza vascular endothelial growth factor (VEGF) ndi kuchepetsa TGF-β1. VEGF ikhoza kuthandizira perifollicular vascularity ndikuthandizira kukula kwa follicle, pamene androgen-inducible TGF-β1 yochokera ku DPCs yakhala ikukhudzidwa ndi kupondereza kufalikira kwa maselo a epithelial.
Pal-AHK Mechanisms ndi Collagen Synthesis
Kafukufuku wa Laboratory ndi Patt et al. akuwonetsa kuti peptide ikhoza kukhala "kumawonjezera kukula ndi kuthekera kwa dermal fibroblasts pomwe kumalimbikitsa kupanga kolajeni” akamangika ku ayoni amkuwa.(5)Dermal fibroblasts ndizomwe zimayambira ma cell a collagen ndi mapuloteni ena owonjezera a matrix, kotero kuti zinthu zochulukirazi komanso zothandiza izi zitha kukhala zofunikira kumtunda kwa ntchito yokonzanso matrix.
Muchitsanzo chofanana cha chikhalidwe cha fibroblast, peptide idawonekeranso kuti imathandizira kutulutsa kwa collagen mtundu I pafupifupi 300% poyerekeza ndi placebo. Izi zikusonyeza kuti Pal-AHK sangangokhala ngati mitogen koma ngati woyambitsa njira ya collagen biosynthesis mu fibroblasts.
Kupitilira collagen, kuphatikizika kwa mkuwa kwa peptide kungathandizire kukonzanso matrix kudzera mu gawo lake ngati cofactor ya lysyl oxidase, enzyme yomwe imayikidwa ku crosslink elastin ndi collagen. Kafukufuku yemwe watchulidwa akuwonetsanso kuti ma peptides amkuwa amatha kuyambitsa matrix metalloproteases (MMPs) ndikulimbikitsa angiogenesis, mwina kudzera pakuwongolera kwa VEGF.
ZINDIKIRANI:Zogulitsazi ndizomwe zimagwiritsidwa ntchito pa kafukufuku wa labotale basi. Peptide iyi sinapangidwe kuti munthu azigwiritsa ntchito. Chonde onaninso ndikutsata zathuMigwirizano ndi zokwaniritsapamaso kuyitanitsa.
Zolozera:
Kapoor R, Shome D, Vadera S, Kumar V, Ram MS. Mapangidwe a QR678 & QR678 Neo Kukula Tsitsi: Phunziro la Ma cell Toxicity & Animal Efficacy Study. Plast Reconstr Surg Glob Open. 2020 Aug 25; 8(8):e2843.doi: 10.1097/GOX.0000000000002843. PMID: 32983753; PMCID: PMC7489598.
Kecel-Gunduza S, Kocb E, Bicaka B, Kokcub Y, Ozela AE, Akyuzc S. IN SILICO KUSANGALALA KWA KUKHALA MAKHALIDWE NDI ZOYENERA ZOYENERA ZA ALA-HIS-LYS (AHK) TRIPEPTIDE. The Online Journal of Science and Technology-July. 2020; 10 (3).
Sadgrove, N. J., & Simmonds, M. S. J. (2021). Zopangira zam'mwamba komanso zopatsa thanzi zatsitsi lathanzi komanso zoletsa kukalamba kwapakhungu pogwiritsa ntchito "zochita ziwiri" (2 kwa 1) ma peptides, mahomoni, ndi cannabinoids. FASEB bioAdvances, 3(8), 601–610.https://doi.org/10.1096/fba.2021-00022
Pyo HK, Yoo HG, Won CH, Lee SH, Kang YJ, Eun HC, Cho KH, Kim KH. Zotsatira za tripeptide-copper complex pakukula kwa tsitsi la munthu mu vitro. Malingaliro a kampani Arch Pharm Res. 2007 Jul; 30 (7): 834-9.doi: 10.1007/BF02978833. PMID: 17703734.
Patt, L. M., & Procyte, A. (2009). Neova® DNA Repair Factor Nourishing Lotion Imalimbikitsa Collagen ndi Speeds Natural Repair Process. khungu, 1, 2
Nthawi yotumiza: 2026-06-02