Kusintha kwa Phossarytion kumathandizanso gawo lofunikira m'miyoyo ndipo limakhudza mbali zingapo. Phossorylation wa mapuloteni amagwirizana kwambiri ndi njira zosiyanasiyana zamankhwala, monga kukonza kuwonongeka kwa DNA, malamulo olemba, komanso malamulo a cell apoptosis. Kuwerenga mapuloteni a phossoryt ndi ma pepturyt kumathandizira kuwulula njira izi ndikukulitsa kumvetsetsa kwathu za ntchito za moyo. Hong Peptide Biotechnology yomwe ili ndi katswiri wokhwima. Kuthana ndi zabwino zake zaukadaulo komanso zowaza, titha kuphatikizira mapepala okhala ndi phosphorted serine (pths), ndi phoshuryt tyrosine mawebusayiti amodzi mpaka asanu ndi asanu.
Mwachidule za matekinoloji a phossillation:
Phossoryt toptuder makamaka amatanthauza mtundu wa ma hydroxyl a serneroxyl a serne (Ser), tyr), tyr), ndi chisokonezo (champando) zosinthidwa kukhala zigawo za acidic. Phossoltot toptudes amatenga gawo lofunikira pophunzira Protein Phossory. Chifukwa chake, ndikofunikira kufufuza mwakuthupi la Phosyryy kwa mapuloteni ndi ma peptides ndikupeza njira zokhazikika komanso zosavuta.
Pakadali pano, njira zosinthira za Phosywalation za ma pepside makamaka zimagwera m'magulu awiri:
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Choyamba, amino acid omwe amafunika kukhala phosphonet (monga mphongo, ser kapena Tyr) amathandizidwa ndi phosphorylation chithandizo. Kenako, kutsatira kaphatikizidwe ka gawo lapansi kokhazikika (SPPS), monooscomet yolumikizidwa ndi malo omwe a Polypeptide. Njirayi ndiyosavuta kuyigwiritsa ntchito ndipo yakhala imodzi mwa njira zazikuluzikulu za phosphortion kusintha kwa ma pepside.
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Mukamagwiritsa ntchito phossary Kuphatikiza apo, kukhazikitsidwa kwa amino acid kudzakhala kovuta kwambiri, makamaka pakakhala masamba angapo a Phosphollat. Njira yophatikizika imakhala yovuta kwambiri, ndipo zomaliza zidzakhala zovuta kwambiri, kupangitsa kulekanitsa kovuta komanso zokolola zochepa kwambiri. Chifukwa chake, pakakhala masamba angapo omwe amafunikira phossorylation mu kulowetsa mu polypepside plandu ya polypeptis motero, kenako phosphorylate mbali imodzi ya hydroxyl ya Ser, Tyr kapena pampando. Panthawi imeneyi, choyamba chotsani unyolo woteteza magulu a amino acid kuti alembedwe. Kwa wopusa ndi mpando osatetezeka a maunyolo omwe amamanga maunyolo atha kuchitika mwachindunji. Mbali yamkunsi yoteteza magulu akhoza kukhala okwanira pansi pa mkhalidwe wa 1% tfa / DCM. Mwanjira imeneyi, gawo logwira ntchito ya Bis-Benzyl Phossomm ndi Tetrazole imatha kupangidwa ndi zomwe zili ndi izi, ndipo zitha kulumikizidwa ndi gulu la hydroxyl. Kenako, gulu la okroadition lomwe lili pansi pa peroxide acid ikhoza kuchitika kuti ipange gulu la phosphonyl, kumaliza phossorylation.
Post Nthawi: 2025-07-16