Chidule cha zomangira zonyansa mu chemistry ndi biology

Mwachilengedwe, cholumikizira cholumikizira chimatanthawuza gulu la R-S-R "RARD. Zogwirizana ndi magulu awiri a Thilol ndi gawo lofunikira mu mawonekedwe a proteinial molekyulu.

Mu ofatsa masiyation njira monga oxidation ndi dMsotion ya DMSO, zokumana nazo monga H2o2, i2, ndi Mercury Stts imatha kugwiritsidwanso ntchito.

Njira ya mpweya:

M'badwo wa zomangira zolumikizirana ndi ma oxidation ndi njira yapamwamba kwambiri mu peptide synthesis, nthawi zambiri ndikusungunuka malo ochepetsedwa m'madzi, pambuyo pa malo osalowerera ndale kapena ofowoka alkaline malo opitilira maola 24. Kuchepetsa kuthekera kwapakatikati kokhazikika, njirayi nthawi zambiri imafunikira kuchitidwa pamalo otsika kwambiri.

Chidule cha zomangira zonyansa mu chemistry ndi biology

Njira Yotsatsa Yayidina:

Ma Peptides adasungunuka mu 25% methanol yankho kapena 30% acetic acid yankho ndi 10mol / l iyodini pa dontho la 15 mpaka 40min. "Pamene peptide utoto umakhala ndi dzina la ayodini, Trp, ndipo pakukumana ndi zotsalira ndi zotsalira zake, ndi mavitamini C kapena sodium thiosulpate nthawi yomweyo kuti muchotse ayodisi owonjezera."

Mphete yopanga njira zambiri zogwirizira zomangira ziwiri

Ngati mndandandawo uli ndi magulu awiri kapena kupitilira awiri omwe amafunikira kuti atulutsidwe, nthawi zambiri pamakhala zochitika ziwiri:

Chilengedwe ndi chopopera mwachisawawa:

CYS idasinthidwa mwachisawawa, ofanana ndi momwe amagwirira ntchito.

Right soint mphete:

Malinga ndi zomwe amapanga, malo omwe atchulidwawa, a CYS, amapanga zomangira zodetsa nkhawa, ndipo njira zomwe zimachitika ndizovuta. Pamaso pa kaphatikizidwe wolimba kwa ma peptides, ndikofunikira kupanga mndandanda ndi njira zopangira magulu angapo ogwirizanitsa, komanso kugwiritsa ntchito mitundu yosiyanasiyana yazakudya pang'onopang'ono kupanga magulu awiri kapena kupitilira apo. Nthawi zambiri amagwiritsa ntchito oteteza maziko ali trt, acm, mmt, tbu, bzl, gulu, magulu osiyanasiyana monga tmob.

Pambuyo popanga njira zopangira zomangira ndi njira ya Cys, tiyenera kutsika pang'ono kuti tidutse chitoliro chilichonse cholumikizidwa ndi chitsimikizo cha chipangizocho. Tsita yotsimikizira ya MS idzaperekedwa limodzi ndi kutumiza.