Zobiriwira komanso zokhazikika za Puti Yophatikiza

Peptide Chessing Synthesis yosankha ndi yolimba gawo la peptide (SPPS), imatha kuvomereza kaphatikizidwe kambiri ka zinthu zingapo za peptide, makamaka zimakhala ndi zinthu zovuta kapena pabwalo la pabwalo. Komabe, zopanga zopanga zomwe zikuwona kaphatikizidwe ka zokambirana zokhazikika makamaka zimavutika chifukwa cha chilengedwe chifukwa chogwiritsa ntchito ma sol sol. Ambiri mwa ma solde omwe amagwiritsidwa ntchito bwino pamankhwala osokoneza bongo omwe amapezeka kale ndi mabungwe azachilengedwe m'maiko osiyanasiyana ndikuyenera kusinthidwa nthawi yomweyo, omwe adalimbikitsa kusungunuka nthawi yomweyo, omwe adalimbikitsa Synthesi Greener. Khama lotereli layang'ana kwambiri chosungunulira, kubwezeretsanso ndikuchepetsa, ndikufufuza njira zogwirizira.

Kubwezera wina kwa peptide ndi kusauka kwake pakamwa, komwe kumachepetsa pakamwa. Kubwezeretsa kumeneku nthawi zambiri kumatha kutumizidwa ndi njira zina zoyendetsera, monga incherana, koma kupita patsogolo kwa zowonjezera, monga kugwiritsa ntchito moled wa molekyulu yofunikayi.

M'zaka zaposachedwa, ma sofnts ambiri omwe amagwiritsidwa ntchito mu Triptide amalota amatanthauzira kuti zikhazikitso zoipitsa ndi echa (kulembetsa, kuwunika kwa mankhwala). Kafukufuku wochulukirapo wa sayansi wayang'ana kwambiri polowa m'Chycl2, DMF ndi NPM mu SPPS. Pofuna kuonetsetsa kuti njira zomwe zakonzedweratu zitha kugwiritsidwa ntchito pakupanga kwakukulu, nkhaniyi sinawoneke kuchokera ku mafakitale opanga mafakitale

Monga kukula ndi zovuta za mankhwalawa zimawonjezeka chaka ndi chaka, kugwiritsa ntchito njira zolaula monga proten ligation kapena ma enzyme cholumikizira zidutswa zazing'ono zowonetsera zitha kukhala zofunika kwambiri kukwaniritsa zosowa zambiri. Ngakhale kusankha kwa njira yolowerera ma peptivesite pamlingo waukulu kumakhala kochepa, zobiriwira komanso mtengo walimbikitsa kukulitsa bwino.

Pomaliza, chitukuko cha ma peptides obiriwira ndilonjeza bwino, makamaka kuti asungunuke. Kuti izi zitheke, takhala tikuwononga ndalama zambiri pakukula kwa nsanja yobiriwira ya Syps ndipo tikuwona nsanja zina za zolosera za zolonda za Peptide, zomwe tidzawunikira pakafunika.