Mwachidule za protein mawu

Ma proteinexpressions amatanthauza ukadaulo wokulitsa majini a chandamale ndikupanga makina olandila ndikuwonetsa mu cell kapena masentimita kuti afotokozere mapuloteni munyengo ya heterolologious dongosolo.

Malingaliro amaloteni amatha kuthetsa vuto lomwe mapuloteni aboma amavuta kupeza. Popeza kukhazikitsidwa koyamba kwa mapuloteni a herterolooloous mu 1970s, mapuloteni masauzande a mapuloteni obwezedwa bwino adafotokozedwa bwino, zomwe zimalimbikitsa kukulitsa chitukuko cha biotechnology ndi kufufuza kwa biomidel.

Cholinga:

Kudzera mwa mapuloteni, ndizotheka kupeza mapuloteni omwe ndi ovuta kupeza kuchokera ku zinthu zachilengedwe.

Kachiwiri, batch yayikulu ndi kuyera kwakukulu kwa mapuloteni mankhwala sikungowonjezera kusanthula mapuloteni, komanso amachepetsa kwambiri mtengo wake.

Chofunika koposa, mapuloteni omwe amasungidwa nthawi zambiri amasankhidwa kuti awonetsere maselo okwera okwera, motero amapewa ngozi zomwe zingatheke.

Njira:

Zimaphatikizapo njira za kubwereza kwa genegment, mawu oyamba m'mawu onena, kutanthauza mawu, kutsuka kwa malonda ndi kusanthula kwa malonda komanso kusanthula.

Mwachidule za protein mawu

Gulu:

Makina opangira mapuloteni amatha kugawidwa mu:

1. Dongosolo la nyukiliya: Esverherizia Coli ndi Protecin yofala kwambiri ya mapuloteni, yomwe ili ndi zabwino zodziwikiratu majini, kubzala, mtengo wotsika komanso mawu okwera. Komabe, limakhala ndikusintha makina omasulira ndipo sioyenera glycosyylation kapena mfundo zapadera zosinthira kusinthitsa ma protein. Bacillus bacillus imatha kugwiritsidwanso ntchito ngati protein mawu, ndipo zabwino zake ndizotheka luso la metabolic ndi ntchito zochepa, zomwe zimakopa chidwi.

2. Zimaphatikizidwadi. ① yisiti mafinya (yisiti yofiira, yisiti yambiri, etc.), yisiti imatha kusinthidwa pambuyo potanthauzira, imakhala ndi ma cell a protein. Dongosolo la ma cell a cell: Majini a Exornuous amapezeka m'matumba a tizilombo ndi bacofururis vector. Ndiwo dongosolo lalikulu la mapuloteni a Eukaryotic, omwe ndi mapuloteni a membrane, mapuloteni a macromalecular, ma protein a protein ndi mapuloteni ena omwe ndi ovuta kufotokoza mu kachitidwe ka Prokaryotic. ③ Maval cell play system: Matembenuzidwe ndi zokongoletsa ndi zokongoletsa za maselo a mammalian ndizokwera kwambiri kuposa edist ndi makina am'manja. Kapangidwe kake ndi ntchito ya maselo a mammalian ali pafupi ndi mapuloteni achilengedwe a Eukaryotic, koma mawu omwe ali otsika ndipo mtengo wake ndi wokwera.

3.