Kukana kwa mankhwala kwa ma peptide kumadalira kapangidwe kake ndi ma amino acid. Ma Peptils a lyophilized nthawi zambiri amakhala olimba kuposa anzawo. Njira zomwe zingachitike kuwonongeka kwa ma peptides ndizotere:
1, Hydrolysis: Nthawi zambiri zimaphatikizidwa mu Asp muzotsatira (d) pazovuta mu peptide, zimakhala zosavuta kupanga siteji yapakati. Mwachitsanzo, pali asp munthawiyo. -Pa (DP) Pankhaniyi, siyiri yooneka bwino yopangidwa ndi acid catalysis imayambitsa cleavage utoto wa peptide. Mofananamo, asy-gly (DG) alipo munthawiyo ndipo poterepo ndi izi apakatikati chitha kuthandizidwa mu mawonekedwe a Asparparte wa Isoaspartecy kapena fanizo lotopa. Pomaliza, ma aspartic onse a aspartic acid amatha kutembenuka kwathunthu ku Isoaspartic acid acid. "Pamlingo wocheperako, umakhala ndi Ser (s), mndandanda womwe umapanganso sider yozungulira yomwe pamapeto pake imatsuka utoto wa peptide."
2, Demomation: Bamu ili movutikira nthawi zambiri imachitika pamaso pa Asn-Gly (NG) kapena GLN-Gly (QG) (QG) motsatizana (DG). Kudzipatula (kuwonongeka kwa Amine) mu Asly-Gly-Gy-Gy-Gy-Gy-Gy-Gy-Gydrelyded wapakati, komwe kumapangitsa kuti apezeke acid asn kapena iyoaspartic acid. Kuphatikiza apo, sizaide-imment itha kuyambitsa D-Asp kapena kuwonongeka kwakunja kuti asunthe-iso - omwe onse angakhale opanda ntchito.
3, oxidation: CYY ndi zotsalira ndi zotsalira zazikulu zomwe zimapangitsa kuti anthu osinthasintha. Kugula kwa Cysteine kumathamangitsidwa pazikhalidwe zapamwamba za pH, komwe Thiol imachotsa mofatsa ndipo imakonda kupanga maunyolo. Chomangira chonyansa chimatha kusinthidwa mosavuta ndi chithandizo cha dithiothreothrel (DTT) kapena Tris (2-Carboxylethylphosphine) hydrochloride (tcerochloride (tcep). Methodine ili ndi mankhwala ndi mankhwala ndi chithunzithunzi kuti mupange methodisine dioxide, yomwe imalowanso methionine dioxide, zomwe ndizovuta kusintha.
4, diketopiprazine ndi pyroglitamic acid njira ya diketopinerazine. Kumbali inayo, ngati Slogn ili ku N-termilu, Pyroglutamic acid sipafupifupi. Izi ndizofanana ndi zomwe nitrogen nayitrogen imaukira mbali ya unyolo wa gloglutathamyl peptide. Kutembenuka kumeneku kumachitikanso ndi N-termisur yomwe ili ndi PYRRGLutate peptide Analogs.
5. Kufananira: Mawuwa amagwiritsidwa ntchito ponena za kutayika kwa kukhulupirika kwa amino acid kapena peptide. Kuthamanga kumaphatikizapo kutembenuka kwa amino acid kuchokera ku base-chothandizira Exantider, nthawi zambiri l-form, kulembera 1 wa L-ndi D-Fomu ijanti. : 1. Kusakaniza. Izi ndizofunikira kwambiri m'badwo wa chilonda, koma ndizosavuta kulowererapo. Kuphatikiza apo, kusinthaku ndikovuta kuzindikira ndipo sikungayang'anitsidwe.
Njira yodziwika kuti mupewe kapena kuchepetsa kuwonongeka kwa peptide ndikuchepetsa peptide ku - 20 ° C kapena bwino - 80 ° C kwa owuma, ngati alipo. Ngati peptide ili mu njira yothetsera matendawa, saquiquo iliyonse iyenera kukhala youndana kuti isawonongedwe ndikuyatsa. Kukhudzana ndi PH & GT kuyenera kupewedwa; Wapakatikati 8. Komabe, ngati pH & gt ikufunika; Ngati peptirira isults pa 8, kukhudzika kwake kuyenera kukhala kochepa.
Post Nthawi: 2025-07-01