Mafunso ndi mayankho okhudza kusungunuka kwa mapepu osinthika

1. Kodi pali zinthu ziti zomwe siziri mu HPLC yoyeretsedwa?

Yankho: Zosintha za peptide komanso zosasinthika zomwe sizisinthidwa: monga kuchuluka kwa peptide-kosinthika ndi zinthu zina zophatikizika monga Dtt, TFA, ndi zina.

2. Kodi ndi zinthu ziti zomwe zimatsukidwa ndi HPLC?

Yankho: Toplc yosinthidwa yoyeretsedwa ndi HPLC idzakhalabe zodetsa zambiri, ndipo zodetsazi nthawi zambiri zimakhala zazifupi komanso tfa yaying'ono.

3. Kodi ndizoyenera nthawi yayitali?

Yankho: kapangidwe ka pepti yosinthidwa iyenera kuganizira kutalika kwa peptide ya pepti yosinthidwa, kulipira, hydrophilicity ndi hydrophobicity ndi zinthu zina. Kutalika kwathunthu, kutsitsa chiyero ndi zipatso zopangidwa ndi zonunkhira, komanso zovuta zapamwamba za kuyeretsedwa ndi kuthekera kwa zovuta mu kapangidwe kake. Komabe, palibe njira yosinthira gawo logwirira ntchito la peptidenti yosinthika, koma kuti amalize kapangidwe ka zino acid omwe angawonjezedwe, nthawi zina kokha kukhazikika kwa madera ogwirira ntchito ndi kutsitsidwa kwa malo ogwirira ntchito ndikusintha kwa malo osinthira. Ngati pepti yosinthidwa ndi yochepa kwambiri, pakhoza kukhala mavuto mu synthesis. Vuto loyamba ndikuti makina osinthika osinthidwa ali ndi zovuta zina pakukonzekera pambuyo pake. Puptive yosinthidwa mkati mwa ma peptides 5 nthawi zambiri imakhala ndi hydrophobic amino acids, apo ayi positi ndizovuta. Zosinthidwa zosinthidwa pansipa 15 amino zotsalira zimatha kupezedwa ndi zokolola zokwanira komanso zokolola.

Mafunso ndi mayankho okhudza kusungunuka kwa mapepu osinthika

4. Momwe mungaweruzire kusungunuka kwa chiwonetsero chosinthidwa kuchokera munjira?

. Amino acid imatha kukhala yovuta kwambiri pakuyeretsa kapena kaphatikizidwe.

(2) the proportion of hydrophobic amino acids under normal conditions < The proportion of charged amino acids can reach up to 20%. If the N or C of the modified peptide is short, the increase of polar amino acids can also improve the solubility.

5. Kodi nchifukwa ninji kuli kovuta kuphatikizira cys, kukwaniritsidwa, kapena trp?

Yankho: Zosinthidwa zokhala ndi ma cys, zomwe zidakumana, kapena ma trp sizingatheke, ndipo zinthu zambiri zoyera ndizovuta kupeza. Izi zimachitika makamaka chifukwa chakuti maguluwa ndi okhazikika komanso amakonda oxidation. Gwiritsani Ntchito ndi Kusunga kwa mapepala osinthidwa amafunikira chisamaliro chapadera kuti muchepetse kutsegulidwa kwa chivindikiro.

6, chifukwa chiyani kupaka mphamvu kapena zoyera zina zikhala zochepa kwambiri?

Yankho: Pali kusiyana kwakukulu pakati pa kapangidwe ka pepala losinthidwa ndi kapangidwe ka Arears. Pali maulemu ochepa omwe sangathe kupangidwa, koma nthawi zambiri pamakhala ma peptives omwe nthawi zambiri sangasinthe. Monga mu Ma Val, Ile, Tyr ndi Trp, Leu, phengu, ma amino amasungunuka osungunuka mu kapangidwe kake kake kameneka. Mphamvu ya kaphatikizidwe ndi kuyera kwa malonda ndi yotsika kwambiri pankhani zotsatirazi, monga: Kubwereza kwa Pro, Ser-Ser-otsatizana, gly, etc.

7. Kodi zimayeretsedwa bwanji?

Yankho: Mapepala osinthidwa nthawi zambiri amayeretsedwa pobwezeretsedwa ndi gawo lotembenuka (monga c88, etc.) pa 214nm. Dongosolo la Buffer nthawi zambiri limakhala yankho lokhala ndi TFA, Ph2.0. Buffer ili ndi 0,1% tfainddddh2o, ndi bufferb yomwe ili ndi 1% tfa / Phn / Ph2.0. Zinasungunuka mu buff musanayeretsedwe; Ngati yankho silikukhutiritsa, sungunulani ndi bufferb, ndiye kuchepetsa ndi buffra; Kwa ma pepticly osinthika osinthika, nthawi zina pang'ono pang'ono kapena acetic acid amawonjezeredwa.