Kutsatira kaphatikizidwe ka peptides pockide-gawo la gawo lolimba, pali njira zambiri za clevage, ndipo mitundu iwiri yofunika tsopano yadziwitsidwa kuti timagwiritsa ntchito.
1. Njira ya Acid Acid (TFA)
Zouma zouma zidayikidwa mu shake flask komanso kuchuluka koyenera komwe kuli kowonjezeredwa. . Tsombuli lidatsitsidwa, kutsukidwa katatu ndi TFE New TFA, kusefedwa kwatsopano, ndipo 8-10 kawiri kuchuluka kwa ayezi wa iltheyl ether. Nthawi zina, tfa ina imatha kusungidwa kuti ipeze gawo lam'mimba. Ether ikhoza kugwiritsidwa ntchito powonjezera mphamvu yozizira ndi kuzizira kwa ice. Pomaliza, chojambula chofufumitsa chakale chimasonkhanitsidwa.
Oyenera Tsimikizani: Wang Resin kapena rink Amide
2, njira yofooka acid (tfa kapena acetic acid dilution) 1% TFA:
1G ya Resin yomwe ili ndi mapepu adawonjezeredwa ku galasi limagwedezeka flask ya dichlororthane yankho la 1% tfa, losindikizidwa ndikugwedezeka kwa mphindi 2. Zosefera zidakanikizidwa ndi nayitrogeni ku methanol quanol flask flask yokhala ndi 10% ya pyrididine, ndipo njirayi idabwerezedwa ka 10. Peptides yotetezayo idatsala pa resin idasambitsidwa katatu pogwiritsa ntchito 30ml ya DCM ndi 30ml wa Meoh. Zotsalira zidayesedwa ndikutsukidwa ndi Tlc kapena HPLC. Mafayilo onse adaphatikizidwa ndipo 5% ya zosungunulira adaphika pansi pa zovuta. Madzi 40 ml amawonjezeredwa ndikuti mpweya wabwino udathandizirana ndi kusakaniza kozizira. Pambuyo pofana, chinthucho chinasambitsidwa katatu ndi madzi oyera, kuyikidwa mu uvuni youma, ndikuwuma ndi koh kapena p2o5.
Dritsani acetic acid:
Pupsides opaka pa resin adapangidwa ndi kudula yankho la 2 h pogwiritsa ntchito Acoh // Tfa / DCM (2: 2: 6), Tsombuli linasambitsidwa, ndipo otumphukira anasambitsidwa katatu ndi yankho la kudula. Asici a acetic owonjezera idachotsedwa ndikuwonjezera khola 15-hexane ku Wosakaniza komanso kuzungulira.
Ntchito Resin: 2-CTC Prin
Post Nthawi: 2025-07-01