Peptide tsopano yakhala zinthu zofunika pazinthu zamankhwala ndipo zikupangidwa pamlingo waukulu. Ma Peptides awa ndi zinthu za biooictive zomwe zimayang'anira ma cell a cell amoyo pamoyo. Kusintha kwa Peptide ndi njira yofunika kwambiri kusintha msana ndi magulu amtundu wa pakhomo la zikhomo zolowera, potero akukhudza chizindikiritso cha zojambulajambula za peptidide. Udindo wa kusintha uku posintha kugwiritsa ntchito makina ogwiritsira ntchito ku Vivo akukhala kofunikira kwambiri. Zoyesa zambiri zawonetsa kuti kugwiritsa ntchito mankhwala osinthika kumatha kuchepetsa kwambiri a immunogenicnicity, kumachepetsa zovuta, kusintha theka la moyo, ndikusintha theka la moyo wa mankhwala osokoneza bongo. Pali njira zambiri zosinthira ma peptur, komanso njira zochepa zomwe zimafotokozedwa mwachidule.
1.Peg peptide zovuta
Pakadali pano moremethoxy polyethylene glycol (Mpeg: Ch2O2 (CH2-Ch2O) N2H) ndiye mtundu wogwiritsidwa ntchito kwambiri wa peptide. Njira yosinthitsira iyi nthawi zambiri imaphatikizapo kuyambitsa magulu a Carboxyl, magulu ena ogwiritsira ntchito kumapeto kwa npembe, kuti akwaniritse mawonekedwe a MPIM,
2. Glycoppides
Glycopmetides, zopangidwa ndi ma Peptides osinthidwa ndi glycosyty, amadziwika kuti glycoptides. Izi glycoppides zimatenga gawo lofunikira pakuwerengera kapangidwe kake ndi ntchito ya glycophteins. Chifukwa chake, kapangidwe ka glycopteride ndi kotsutsa kwambiri. Pakadali pano, kulumikizana pakati pa oligosaccharide ndi polypeptide kumadutsa c, n, o, ndi s glycosidic, ndi o-glycosidic omwe amagwiritsidwa ntchito kwambiri. Ma bond a glycosidic osakhazikika amalimbikitsa kwambiri glycosidic kwambiri amawonjezera zovuta za peptidis. "Ma bond a glycosiidic awa amangokhala hydrolyzid m'malo a acidic, ndi kwa onse a serine a glycosy, omwe ali pachiwopsezo, pamakhala kuthekera kwa kusintha kwa ma β-kuchotsedwanso pang'ono."
3. Phosphopmeptide
Phossorlation ndi Dephsvory, zomangamanga zimapezeka pafupifupi nthawi zonse za zochitika m'moyo, kuphatikiza kuchuluka kwa maselo, kukula, kusiyanasiyana, kusiyanasiyana kwa minofu, metabolism ndi kutupa. Pakati pawo, phosphopueptides ndi zitsanzo zabwino kwambiri kuti muwonetsetse kusintha kwa ma prosphorlat. Malinga ndi amino atsalira omwe ali phosphoryted, mapfortol topsides amatha kulembedwa m'makalasi anayi: o-phssested chimati, acyl phosphopeptides, ndi s-phosphooptides. O-phosstoylated maptides amapangidwa ndi phosroryl wa ma amino acid monga a rino, chisokonezo, tydroxypline kapena hydroxylysine; N-phossovettlated mapeptides amachokera ku phosphorylation wa arginine, lysine kapena hirbidine; Acyl-phosphopueptides amapangidwa ndi phosphorylation ya aspartate kapena glutamate; Mosiyana ndi izi, s-phossowt toptides amapangidwa ndi phosphorylation ya Chymeine.
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Laptus a Cyclics amatha kugawidwa m'malime awiri: homacycclic pamalonda ndi amino acid olumikizidwa ndi zomangira; Wina ndi hereocyclic peptirira, omwe mawonekedwe ake amalumikizana ndi ester, ma elther zomangira, zomangira za Thioester ndi zolumikizira zomangira kuphatikiza kwa amayi.
Ma Peptider afupiafupi amasokonekera mosavuta miyambo yosiyanasiyana ya michere yachilengedwe ku Vivo, ndikupanga mapangidwe a cyclic amatha kukulitsa enzymatic ndi kukhazikika kwa mankhwala a Peptides. Popeza ma paptic a cycli amalibe c ndi n tempyi, amatha kuchepetsa kuwonongeka kwa Aminoptidase ndi Carboxypeptidase, potero kumawonjezera luso la peptymatic hydrolysic hyrolysis. Nthawi yomweyo, mapangidwe a mphete amalepheretsa kusintha kwa magwiridwe, komwe kumawonjezera ubale ndi kusankhidwa pakati pa peptide ndi wolandila, kukonza zochitikazo ndikuchepetsa zoyipazo. Chifukwa chake, lakhala malangizo atsopano a chitukuko chatsopano cha mankhwala m'zaka zaposachedwa.
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Makina owoneka bwino ophatikizidwa ndi maluso oyerekeza angagwiritsidwe ntchito kuzindikira zomwe akufuna. Mu lingaliro la vitro pogwiritsa ntchito microccopy kapena flurescence microctopy amakhalabe njira imodzi yothandiza kwambiri yophunzirira njira zingapo zachilengedwe ndi zogwirizanitsa mkati mwa maselo. Mapulogalamu awa, mosiyana ndi mapuloteni enieni, omwe amakhala ndi zofuna za Actin ndipo sakonda kuphatikizika kwa protein, kuwapangitsa kukhala oyenera kutsata vitro. Kuphatikiza apo, cell yolumikizidwa ndi ceptide yolowera (CPP) imatha kugwiritsidwanso ntchito pazithunzi za intracellular ndi cytotoxicity.
Kwa nthawi yayitali, fetre tikulimbikitsidwa kuti azisintha. Fluorescence Resonance Enernation (Fret) ndi makina ofotokoza mphamvu yosamutsa pakati pa awiri a fluoropengores. Chifukwa kuchita bwino kumatengera gawo pakati pa wopereka ndi mamolekyu ovomerezeka, njirayi nthawi zambiri imagwiritsidwa ntchito pophunzira ezyme
Post Nthawi: 2025-07-01