Zomwe zingakhale zokhudzana ndi kuwonongeka kwa polypeptis synthesis

Mankhwala amatha kuthandizidwa ndi acid, zitsulo, ma proteases kapena zitsulo ma ion pansi pamikhalidwe ina. "Mwachitsanzo, theka la moyo wa hydrolysis of the dlycylglycine mu 1mol / lnaoh ndi pafupifupi masiku 25,000 mu 1mol / l hydrochloric acid." Komabe, ma boti ogwirira ntchito ogwirira ntchito omwe amagwira ntchito paltadium amatha kutsukidwa mwachangu ndi palladium ndi ma courpecs. Ndiye ndi zinthu ziti zomwe zikukhudzidwa mu hydrolysis papepala la Peptide?

Zomwe zingakhale zokhudzana ndi kuwonongeka kwa polypeptis synthesis

1, pH

Mankhwalawa amakhazikika mosavuta mu acidic ndi alkaline malo, komanso kuchuluka kwa hydrolysis kumakhala kochepa kwambiri. Hydrolysis a C-termine glycine ya tetrapeptide idayesedwa pa PH 0 mpaka 14 Dera pa 25 ° C, ndipo ubale pakati pa Hydrolysis Sydiction ndi zokongoletsera. Pakati pawo, ma hydrolysis a peptide pa PH = 7 anali 3 × 10-9s-1, ndipo theka la moyo wake adawerengedwa kuti akhale zaka 7.

2. Mitundu ya kutentha

Mankhwala a hydrolysis a mankhwalawa kutentha ndi mwachiwonekere kuposa momwe firiji, ndipo kusiyana pakati pa awiriwa ndi kwakukulu. Kuyesayesa kwasayansi kwatsimikizira za hydrolysis ya tetraptides mu ozizira komanso otentha. Ngakhale hydrolysis ya geovermal stun yakhala yoposa ya ozizira pang'onopang'ono, imakhalabe yokhazikika pambuyo pa 1500min. Mtengo wa Hydrolysis wa 50 ° C ndi 25 ° C ATHANDIZA MTIMA WA HYDROlysis pa 150

3. Enzyme catalytics

Zomangira zolumikizira pamasamba osiyanasiyana a polypeptide mankhwala zimafunikira hydrolyzered ndi ma enzymes osiyanasiyana komanso kutentha kosiyanasiyana, komanso kuchuluka kwa hydrolysis pamalo osiyanasiyana ndikosiyananso. Zotsatira zake zidawonetsa kuti ma hydrolysis muyeso ndi theka-moyo wa chiwonetsero cha C-termide omwe ali ndi Carboxypeptidad B, chomangira chamkati chokhala ndi enzyme, ndi madandaulo ascitic chotupa. Kuphatikiza apo, kuchuluka kwa magulu atatu a Peptide popanda enzymatic catalysis ndi hydrolysis chiwongolero cha enzymatic pambuyo pa catalysis adasanthudwa. Pomaliza, adalipo kuti Carboxypeptivese B anali alzyme kwambiri pakati pa magulu atatu a ma enzymes.